PXD034899-1

PXD034899 is an original dataset announced via ProteomeXchange.

Title	Acetylation of p62 regulates base excision repair through interaction with APE1
Description	p62, a well-known adaptor of autophagy, plays multiple functions in response to various stresses. Here, we report a function for p62 in base excision repair that is distinct from its known functions. Loss of p62 impairs BER capacity and increases the sensitivity of cancer cells to alkylating and oxidizing agents. In response to alkylative and oxidative damage, p62 is accumulated in the nucleus，acetylated by hMOF， deacetylated by SIRT7, and acetylated p62 is recruited to chromatin. The chromatin-enriched p62 directly interacts with APE1, a key enzyme of the BER pathway, and promotes its endonuclease activity, which facilitates BER and cell survival. Collectively, our findings demonstrate that p62 is a regulator of BER, and provide further rationale for targeting p62 as a cancer therapeutic strategy.
HostingRepository	PRIDE
AnnounceDate	2022-07-19
AnnouncementXML	Submission_2022-07-18_19:17:36.110.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meiting Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-26 07:30:44	ID requested
⏵ 1	2022-07-18 19:17:36	announced
2	2023-11-14 08:56:09	announced	2023-11-14: Updated project metadata.

10.1016/J.CELREP.2022.111116;

submitter keyword: Base excision repair, p62, Acetylation, hMOF, SIRT7, APE1

Haiying Wang
contact affiliation	Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Department of Biochemistry and Biophysics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.
contact email	wendy@bjmu.edu.cn
lab head
Meiting Li
contact affiliation	Peking University Health Science Center
contact email	Limeiting401@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034899

PRIDE project URI

• PRIDE
  1. PXD034899
     1. Label: PRIDE project
     2. Name: Acetylation of p62 regulates base excision repair through interaction with APE1