PXD034895 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The augmin complex architecture reveals structural insights into microtubule branching |
Description | In mitosis, the augmin complex binds to spindle microtubules to recruit the γ-tubulin ring complex (γ-TuRC), the principal microtubule nucleator, for the formation of branched microtubules. Our understanding of augmin-mediated microtubule branching is strongly hampered by the lack of structural information on the augmin complex. Here we elucidate the molecular architecture and define the conformational plasticity of the augmin complex using an integrative structural biology approach. The elongated structure of the augmin complex is characterised by extensive coiled-coil segments and comprises two structural elements with distinct but complementary functions in γ-TuRC and microtubule binding, linked by a flexible hinge. The augmin complex is recruited to microtubules via a composite microtubule binding site comprising a positively charged unordered extension and two Calponin homology domains. Our study provides the structural basis for augmin function in branched microtubule formation, decisively fostering our understanding of spindle formation in mitosis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:14:29.494.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Erik Zupa |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-26 07:29:00 | ID requested | |
1 | 2022-10-13 03:51:13 | announced | |
⏵ 2 | 2023-11-14 07:14:30 | announced | 2023-11-14: Updated project metadata. |
Publication List
Zupa E, W, ü, rtz M, Neuner A, Hoffmann T, Rettel M, B, ö, hler A, Vermeulen BJA, Eustermann S, Schiebel E, Pfeffer S, The augmin complex architecture reveals structural insights into microtubule branching. Nat Commun, 13(1):5635(2022) [pubmed] |
Keyword List
submitter keyword: microtubule, augmin, nucleation, cell cycle, branching |
Contact List
Stefan Pfeffer |
contact affiliation | Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany |
contact email | s.pfeffer@zmbh.uni-heidelberg.de |
lab head | |
Erik Zupa |
contact affiliation | Heidelberg University |
contact email | e.zupa@zmbh.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034895
- Label: PRIDE project
- Name: The augmin complex architecture reveals structural insights into microtubule branching