PXD034894-3

PXD034894 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Integrative structure determination of an 85-kilodalton PTBP1-IRES complex in solution reveals a strong compaction with pronounced conformational flexibility
Description	Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the interaction between the four RNA recognition motifs (RRMs) of the protein PTBP1 with the internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV). These results are a reanalysis of PXD005566, with a broader parameter set.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:07:24.884.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Sarnowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-26 07:28:43	ID requested
1	2023-10-24 07:07:15	announced
2	2023-11-14 09:06:20	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:07:25	announced	2024-10-22: Updated project metadata.

Publication List

10.1038/s41467-023-42012-z;
Dorn G, Gmeiner C, de Vries T, Dedic E, Novakovic M, Damberger FF, Maris C, Finol E, Sarnowski CP, Kohlbrecher J, Welsh TJ, Bolisetty S, Mezzenga R, Aebersold R, Leitner A, Yulikov M, Jeschke G, Allain FH, Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility. Nat Commun, 14(1):6429(2023) [pubmed]

10.1038/s41467-023-42012-z;

Dorn G, Gmeiner C, de Vries T, Dedic E, Novakovic M, Damberger FF, Maris C, Finol E, Sarnowski CP, Kohlbrecher J, Welsh TJ, Bolisetty S, Mezzenga R, Aebersold R, Leitner A, Yulikov M, Jeschke G, Allain FH, Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility. Nat Commun, 14(1):6429(2023) [pubmed]

Keyword List

submitter keyword: UV cross-linking
PTBP1
IRES
RRM
splicing
structural proteomics
protein-RNA
RNA-protein
protein-RNA complex
RNA-protein complex
CLIR-MS
integrative modelling
structure

submitter keyword: UV cross-linking

PTBP1

IRES

RRM

splicing

structural proteomics

protein-RNA

RNA-protein

protein-RNA complex

RNA-protein complex

CLIR-MS

integrative modelling

structure

Contact List

Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
lab head
Christopher Sarnowski
contact affiliation	DISCO Pharmaceuticals SWISS GmbH
contact email	sarnowski@imsb.biol.ethz.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD034894
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD034894

PRIDE project URI

Repository Record List

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