PXD034894 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrative structure determination of an 85-kilodalton PTBP1-IRES complex in solution reveals a strong compaction with pronounced conformational flexibility |
Description | Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the interaction between the four RNA recognition motifs (RRMs) of the protein PTBP1 with the internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV). These results are a reanalysis of PXD005566, with a broader parameter set. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:06:12.325.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christopher Sarnowski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-26 07:28:43 | ID requested | |
1 | 2023-10-24 07:07:15 | announced | |
⏵ 2 | 2023-11-14 09:06:20 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dorn G, Gmeiner C, de Vries T, Dedic E, Novakovic M, Damberger FF, Maris C, Finol E, Sarnowski CP, Kohlbrecher J, Welsh TJ, Bolisetty S, Mezzenga R, Aebersold R, Leitner A, Yulikov M, Jeschke G, Allain FH, Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility. Nat Commun, 14(1):6429(2023) [pubmed] |
Keyword List
submitter keyword: UV cross-linking |
PTBP1 |
IRES |
RRM |
splicing |
structural proteomics |
protein-RNA |
RNA-protein |
protein-RNA complex |
RNA-protein complex |
CLIR-MS |
integrative modelling |
structure |
Contact List
Alexander Leitner |
contact affiliation | ETH Zurich, Institute of Molecular Systems Biology |
contact email | leitner@imsb.biol.ethz.ch |
lab head | |
Christopher Sarnowski |
contact affiliation | Institute of Molecular Systems Biology, Department of Biology, ETH Zürich |
contact email | sarnowski@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD034894 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034894
- Label: PRIDE project
- Name: Integrative structure determination of an 85-kilodalton PTBP1-IRES complex in solution reveals a strong compaction with pronounced conformational flexibility