PXD034880-1
PXD034880 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulation of urea cycle by reversible high stoichiometry lysine succinylation |
Description | The posttranslational modification, lysine succinylation is implicated in the regulation of various metabolic pathways. However, its biological relevance remains uncertain due to methodological difficulties in determining high impact succinylation sites. In the present study, using stable isotope labeling and data independent acquisition mass spectrometry, we quantified lysine succinylation stoichiometries in mouse livers. Despite the low overall stoichiometry of lysine succinylation, several high stoichiometry sites were identified, especially upon deletion of the desuccinylase SIRT5. In particular, multiple high stoichiometry lysine sites identified in argininosuccinate synthase ASS1, a key enzyme in urea cycle, are regulated by SIRT5. Mutation of the high stoichiometry lysine in ASS1 to succinyl mimetic glutamic acid significantly decreased its enzymatic activity. Metabolomics profiling confirms that SIRT5 deficiency decreases urea cycle activity in liver. Importantly, SIRT5 deficiency compromises ammonia tolerance and reduces locomotor and exploratory activity in male mice upon high ammonium diet feeding. Therefore, lysine succinylation is functionally important in ammonia metabolism. |
HostingRepository | MassIVE |
AnnounceDate | 2023-12-08 |
AnnouncementXML | Submission_2023-12-08_15:34:21.209.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Birgit Schilling |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | N6-succinyl-L-lysine |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-06-24 15:53:27 | ID requested | |
⏵ 1 | 2023-12-08 15:34:21 | announced |
Publication List
no publication |
Keyword List
submitter keyword: data independent acquisition, sirtuin |
Contact List
Birgit Schilling | |
---|---|
contact affiliation | Buck Institute |
contact email | bschilling@buckinstitute.org |
lab head | |
Birgit Schilling | |
contact affiliation | Buck Institute |
contact email | bschilling@buckinstitute.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089726/ |