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PXD034880-1

PXD034880 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRegulation of urea cycle by reversible high stoichiometry lysine succinylation
DescriptionThe posttranslational modification, lysine succinylation is implicated in the regulation of various metabolic pathways. However, its biological relevance remains uncertain due to methodological difficulties in determining high impact succinylation sites. In the present study, using stable isotope labeling and data independent acquisition mass spectrometry, we quantified lysine succinylation stoichiometries in mouse livers. Despite the low overall stoichiometry of lysine succinylation, several high stoichiometry sites were identified, especially upon deletion of the desuccinylase SIRT5. In particular, multiple high stoichiometry lysine sites identified in argininosuccinate synthase ASS1, a key enzyme in urea cycle, are regulated by SIRT5. Mutation of the high stoichiometry lysine in ASS1 to succinyl mimetic glutamic acid significantly decreased its enzymatic activity. Metabolomics profiling confirms that SIRT5 deficiency decreases urea cycle activity in liver. Importantly, SIRT5 deficiency compromises ammonia tolerance and reduces locomotor and exploratory activity in male mice upon high ammonium diet feeding. Therefore, lysine succinylation is functionally important in ammonia metabolism.
HostingRepositoryMassIVE
AnnounceDate2023-12-08
AnnouncementXMLSubmission_2023-12-08_15:34:21.209.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBirgit Schilling
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListN6-succinyl-L-lysine
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-24 15:53:27ID requested
12023-12-08 15:34:21announced
Publication List
no publication
Keyword List
submitter keyword: data independent acquisition, sirtuin
Contact List
Birgit Schilling
contact affiliationBuck Institute
contact emailbschilling@buckinstitute.org
lab head
Birgit Schilling
contact affiliationBuck Institute
contact emailbschilling@buckinstitute.org
dataset submitter
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Dataset FTP location
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