PXD034875-2

PXD034875 is an original dataset announced via ProteomeXchange.

Title	Structure of the human FERRY Rab5 effector complex
Description	Long-range mRNA transport is crucial for the spatio-temporal regulation of gene expression, and its malfunction is linked to neurological disorders. The pentameric FERRY Rab5 effector complex is the molecular link between mRNA and the early endosome in mRNA intracellular distribution. Here, we determine the cryo-EM structure of the human FERRY complex, composed of Fy-1 to Fy-5. The structure reveals a clamp-like architecture, in which two arm-like appendages, each consisting of Fy-2 and a Fy-5 dimer, protrude from the central Fy-4 dimer. We demonstrate that the coiled-coil domains of Fy-2 are flexible and project into opposite directions from the FERRY complex core. While the C-terminal coiled-coil acts as binding region for Fy-1/3 and Rab5, both coiled-coils together with Fy-5 bind mRNA. Thus, Fy-2 serves as binding hub that connects not only all five complex subunits, but also mediates the binding to mRNA and to the early endosome via Rab5. The FERRY structure provides novel mechanistic insight into long-distance mRNA transport.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:13:33.241.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Welp
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-24 08:45:53	ID requested
1	2023-10-23 04:29:09	announced
⏵ 2	2023-11-14 08:13:33	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, structure

Henning Urlaub
contact affiliation	Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany Institute of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Luisa Welp
contact affiliation	Max-Planck-Institute for Biophysical Chemistry
contact email	lwelp@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034875
     1. Label: PRIDE project
     2. Name: Structure of the human FERRY Rab5 effector complex