PXD034873-1

PXD034873 is an original dataset announced via ProteomeXchange.

Title	Characterization of the diphthamide modification of eEF2 in Arabidopsis thaliana
Description	Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target of diphtheria toxin. Diphthamide biosynthesis depends on the 4Fe-4S-cluster protein Dph1 catalyzing the first committed step, as well as Dph2 to Dph7, in yeast and mammals. Here we show that diphthamide modification of eEF2 is conserved in Arabidopsis thaliana and requires AtDPH1. Ribosomal -1 frameshifting-error rates are increased in Arabidopsis dph1 mutants, similar to yeast and mice. Compared to the wild type, shorter roots and smaller rosettes of dph1 mutants result from fewer formed cells. TARGET OF RAPAMYCIN (TOR) kinase activity is attenuated, and autophagy is activated, in dph1 mutants. Under abiotic stress diphthamide-unmodified eEF2 accumulates in wild-type seedlings, most strongly upon heavy metal excess, which is conserved in human cells. In summary, our results suggest that diphthamide contributes to the functionality of the translational machinery monitored by plants to regulate growth.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_04:41:14.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorenz Adrian
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-24 08:45:20	ID requested
⏵ 1	2022-08-11 04:41:15	announced

Zhang H, Quintana J, Ü, tk, ü, r K, Adrian L, Hawer H, Mayer K, Gong X, Castanedo L, Schulten A, Janina N, Peters M, Wirtz M, Brinkmann U, Schaffrath R, Kr, ä, mer U, Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis. Nat Commun, 13(1):4009(2022) [pubmed]

submitter keyword: Diphthamide TRANSLATION ELONGATION FACTOR 2 (eEF2) Arabidopsis thaliana Growth Development

Ute Kraemer
contact affiliation	Molecular Genetics and Physiology of Plants, Ruhr University Bochum, Germanz
contact email	Ute.Kraemer@ruhr-uni-bochum.de
lab head
Lorenz Adrian
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	lorenz.adrian@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034873
     1. Label: PRIDE project
     2. Name: Characterization of the diphthamide modification of eEF2 in Arabidopsis thaliana