PXD034831 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Response of Pseudomonas aeruginosa laboratory strain PAO1 and clinical isolates LESlike1, LESB58, and LES431 to antibiotic treatments |
Description | Pseudomonas aeruginosa is a predominant pathogen in chronic lung infections in individuals with cystic fibrosis (CF). Epidemic strains of P. aeruginosa, such as the Liverpool Epidemic Strain (LES), are capable of transferring between CF patients and have been associated with increased hospital visits and antibiotic treatments. We used label-free quantitative proteomics to compare the laboratory strain PAO1, beta-lactam resistant isolate LESB58, and beta-lactam susceptible isolate LESlike1 and their responses to three beta-lactams (aztreonam, carbenicillin, piperacillin), the aminoglycoside tobramycin, and hydrogen peroxide. Across all samples, we identified 3019 proteins with a minimum of two peptides. We found that LESB58 showed a large response to treatment with the beta-lactam carbenicillin, with 644 proteins significantly increased in abundance and 590 proteins significantly decreased in abundance (Students t-test, p≤0.05, FDR=0.05, S0=1). Proteomic characterization of an additional beta-lactam resistant isolate, LES431, exposed to carbenicillin showed that this response was shared by both isolates. Part of the response to carbenicillin in LESB58 included an increase in abundance in proteins involved in cell wall synthesis and division. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:10:26.781.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cezar Khursigara |
SpeciesList | scientific name: Pseudomonas aeruginosa LES431; NCBI TaxID: 1408272; scientific name: Pseudomonas aeruginosa LESlike1; NCBI TaxID: 1408274; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: NCBITaxon:208964; scientific name: Pseudomonas aeruginosa LESB58; NCBI TaxID: 557722; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-22 02:31:46 | ID requested | |
1 | 2023-08-23 09:27:14 | announced | |
⏵ 2 | 2023-11-14 07:10:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: antibiotics, label-free quantitative proteomics, Liverpool Epidemic Strain,Pseudomonas aeruginosa |
Contact List
Cezar Khursigara |
contact affiliation | University of Guelph, Department of Molecular and Cellular Biology |
contact email | ckhursig@uoguelph.ca |
lab head | |
Cezar Khursigara |
contact affiliation | University of Guelph |
contact email | ckhursig@uoguelph.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD034831 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034831
- Label: PRIDE project
- Name: Response of Pseudomonas aeruginosa laboratory strain PAO1 and clinical isolates LESlike1, LESB58, and LES431 to antibiotic treatments