PXD034635-1
PXD034635 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein nonadditivity and stability contribute to heterosis in Arabidopsis hybrids and allotetraploids |
| Description | Hybrid vigor or heterosis has been widely applied in agriculture and extensively studied at the genetic and gene expression levels. However, the biochemical mechanism underlying heterosis remains elusive. One theory suggests that a decrease in protein aggregation may occur in hybrids due to the presence of protein variants between parental alleles, but it has not been experimentally tested. Here, we report comparative analysis of soluble and insoluble proteomes in Arabidopsis intraspecific and interspecific hybrids or allotetraploids formed between A. thaliana and A. arenosa. Both intraspecific hybrids and interspecific allotetraploids displayed non-additivity of the expressed proteins, including many biotic and abiotic stress-responsive proteins. In the allotetraploids, homoeolog-expression bias was not observed among all proteins examined but could occur among 17-20% of the nonadditively expressed proteins with more A. thaliana-biased than A. arenosa-biased homoeologs, consistent with the transcriptome results. Analysis of the insoluble and soluble proteomes revealed more soluble proteins in the hybrids than their parents but not in allotetraploids. Most proteins in ribosomal biosynthesis and in the thylakoid lumen, membrane, and stroma, were in the soluble fractions, indicating a role of protein stability in photosynthetic activities for promoting growth. Together, these results support roles for nonadditive expression of stress-responsive proteins and reduction of protein biosynthesis in mediating heterosis in Arabidopsis hybrids and allotetraploids. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-07-07 |
| AnnouncementXML | Submission_2023-07-07_19:21:27.565.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Viviana June |
| SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; scientific name: Arabidopsis suecica; NCBI TaxID: 45249; scientific name: Arabidopsis arenosa; NCBI TaxID: 38785; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-06-17 10:01:27 | ID requested | |
| ⏵ 1 | 2023-07-07 19:21:27 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Hybrid vigour, Heterosis, Arabidopsis, Allopolyploids, Protein solubility |
Contact List
| Z. Jeffrey Chen | |
|---|---|
| contact affiliation | University of Texas at Austin |
| contact email | zjchen@austin.utexas.edu |
| lab head | |
| Viviana June | |
| contact affiliation | UT Austin |
| contact email | vivianajune@utexas.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089682/ |
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