PXD034615-1

PXD034615 is an original dataset announced via ProteomeXchange.

Title	BRD4 regulates the osteogenic differentiation of MSCs
Description	MSCs were lysed in cell lysis buffer for Western blotting and IP (Beyotime, Cat. No. P0013). A Dynabeads™ protein G immunoprecipitation kit (Invitrogen, Cat. No. 10007D) was used for Co-IP according to the manufacturer’s instructions. Dynabeads were resuspended and placed on a magnet to remove the supernatant then incubated with Ab Binding & Washing Buffer containing an anti-BRD4 antibody for 10 min at room temperature with rotation. The supernatant was removed by placing tubes on a magnet, and the Dynabeads-antibody complex was washed with Ab Binding & Washing Buffer. MSC lysates were added to the Dynabeads-antibody mixture and incubated for 10 min at room temperature with rotation. The supernatant was removed, the Dynabeads-antibody complex mixture was washed with washing buffer, and the proteins were separated using a standard SDS–PAGE system.
HostingRepository	iProX
AnnounceDate	2022-06-16
AnnouncementXML	Submission_2023-01-03_01:46:59.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenhui Yu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 18:45:28	ID requested
⏵ 1	2023-01-03 01:46:59	announced
2	2023-06-11 20:13:24	announced	2023-06-12: Update publication information.

Dataset with its publication pending

submitter keyword: MSCs

Huiyong Shen
contact affiliation	The Eighth Affiliated Hospital of Sun Yat-sen University
contact email	shenhuiy@mail.sysu.edu.cn
lab head
Wenhui Yu
contact affiliation	The Eighth Affiliated Hospital of Sun Yat-sen University
contact email	yuwh5@mail2.sysu.edu.cn
dataset submitter

iProX dataset URI