PXD034604-1

PXD034604 is an original dataset announced via ProteomeXchange.

Title	Cardioviruses’ leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic
Description	Proteins from unrelated pathogens, including some RNA or DNA viruses and bacteria can recruit and activate cellular p90-ribosomal protein S6 kinases (RSKs) through a common linear motif. Data suggested a model where pathogens' proteins act to dock the recruited RSKs toward specific substrates, which then act as effectors to the benefit of the pathogens. Using cardiovirus leader protein (L) as a paradigm, we show that pathogens' proteins can modify the spectrum of RSK substrates in infected cells. L triggers nucleocytoplasmic trafficking perturbation and phenylalanine-glycine (FG)-nucleoporin hyperphosphorylation in an RSK-dependent fashion. Biotin ligase experiments identified FG-nucleoporins as common partners of L and RSK in infected cells. Using cells expressing an analog-sensitive RSK2 mutant, we show that L triggers direct phosphorylation of NUP98 and NUP214 by RSK2 in infected cells. Our data therefore demonstrate a novel virulence mechanism where pathogens' proteins hijack and retarget cellular protein kinases of the RSK family
HostingRepository	PRIDE
AnnounceDate	2022-12-08
AnnouncementXML	Submission_2022-12-08_01:25:37.944.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DidierVertommen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 03:51:42	ID requested
⏵ 1	2022-12-08 01:25:38	announced
2	2023-11-14 08:57:22	announced	2023-11-14: Updated project metadata.

submitter keyword: p90 ribosomal S6 kinases, Picornavirus, nucleocytoplasmic traffic, RSK, cardiovirus, phosphorylation, YopM, nucleoporins,linear motifs

ThomasMichiels
contact affiliation	VIRO unit, de Duve Institute, Université Catholique de Louvain, Brussels, 1200, Belgium
contact email	thomas.michiels@uclouvain.be
lab head
DidierVertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034604
     1. Label: PRIDE project
     2. Name: Cardioviruses’ leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic