PXD034562-1

PXD034562 is an original dataset announced via ProteomeXchange.

Title	Deciphering the involvement of the Hippo pathway co - regulators, YAP/TAZ in invadopodia formation and matrix degradation
Description	Invadopodia are adhesive, actin - rich pro trusions , formed by metastatic cancer cells , that degrade the extracellular matrix and facilitate invasion . They support the metastatic cascade by a spatially and temporally coordinated process where by invading cells bind to the matrix , degrade it by speci fic metalloproteinases , and mechanically penetrate diverse tissue ba rriers by forming actin - rich extensions . However, despite the apparent involvement of invadopodia in the metasta t i c process , the molecular mechanisms that regulate invadopodia formation an d function are still largely unclear. In this study, we have explored the involvement of the key Hippo pathway co - regulators , namely YAP , and TAZ , in invadopodia formation and matrix degradation. Towards that goal, we tested the effect of depletion of YAP, TAZ , or both on invadopodia formation and activity in multiple human cancer cell lines. We report that knockdown of YAP and TAZ or their inh ibit ion by verteporfin indu ce a significant elevation in matrix degradation and invadopodia formation in several ca ncer cell lines . Conversely , o verexpression of these proteins strongly suppress es invadopodia formation and matrix degradation . Proteomic and transcriptomic profiling of MDA - MB - 231 cells , following co - knockdown of YAP and TAZ , revealed a significant change in the levels of key invadopodia - associated proteins, including the crucial proteins Tks5 and MT1 - MMP (MMP14). Collectively, our findings show that YAP and TAZ act as negative regulators of invadopodia formation in diverse cancer lines, most likely by red ucing the levels of essential invadopodia components. Dissecting the molecular mechanisms of invadopodia formation in cancer invasion may eventually reveal novel targets for therapeutic applications against invasive cancer
HostingRepository	PRIDE
AnnounceDate	2023-03-20
AnnouncementXML	Submission_2023-03-20_11:29:37.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DavidMorgenstern
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 02:22:34	ID requested
⏵ 1	2023-03-20 11:29:38	announced

Dataset with its publication pending

submitter keyword: prote omic profiling , Tks5, extracellular matrix , YAP ,Hippo pathway , i nvadopodia, actin, TAZ , gelatin degradation, cancer metastasis, MMP14 (MT1 - MMP) , RNA sequencing

BenjaminGeiger
contact affiliation	Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel
contact email	benny.geiger@weizmann.ac.il
lab head
DavidMorgenstern
contact affiliation	Weizmann institute
contact email	david.morgenstern@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034562

PRIDE project URI

• PRIDE
  1. PXD034562
     1. Label: PRIDE project
     2. Name: Deciphering the involvement of the Hippo pathway co - regulators, YAP/TAZ in invadopodia formation and matrix degradation