PXD034537-3

PXD034537 is an original dataset announced via ProteomeXchange.

Title	Convergence of distinct RNA-silencing pathways on GW182/Tnrc6
Description	The RNA-binding protein Trim71/Lin41 is a phylogenetically conserved developmental regulator that functions in mammalian stem cell reprogramming, brain development and cancer. Trim71 recognizes target mRNAs through hairpin motifs and silences them through molecular mechanisms that await identification. Deposited data are mass spectrometry data from immunoprecipitation experiments with endogenously tagged Trim71, Ago2, and Tnrc6a. Experiments are carried out in mouse embryonic stem cells (mESCs) and uncover reciprocal interactions of Trim71, Ago2, and Tnrc6a. Trim71 protein interactions are largely independent of Ago2 levels, but strongly depend on the presence of RNA. A second set of experiments is mass spectrometry data from immunoprecipitation experiments in mESCs overexpressing a tagged peptide derived from Homo sapiens TNRC6B (‘FLAG-HA::T6BWT::Cherry’), that is known to block the Tnrc6-Ago2 interaction. We show that a wild-type version strongly binds Ago1 and Ago2, but not Trim71, while a mutant version binds neither Ago1, 2, nor Trim71.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:52:08.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Hess
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-15 05:13:10	ID requested
1	2023-07-04 06:56:09	announced
2	2023-11-14 07:03:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:52:09	announced	2024-10-22: Updated project metadata.

10.1016/J.MOLCEL.2023.06.001;

submitter keyword: TRIM71

LIN41

RNA-binding protein

stem cell

miRNA

AGO2

GW182

miRISC

TNRC6

post-transcriptional gene regulation

Helge Grosshans
contact affiliation	Multicellular Systems Friedrich Miescher Institute for Biomedical Research
contact email	helge.grosshans@fmi.ch
lab head
Daniel Hess
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	dhess@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD034537

PRIDE project URI

• PRIDE
  1. PXD034537
     1. Label: PRIDE project
     2. Name: Convergence of distinct RNA-silencing pathways on GW182/Tnrc6