PXD034490-1

PXD034490 is an original dataset announced via ProteomeXchange.

Title	The Legionella-driven PtdIns(4)P gradient at LCV-ER membrane contact sites promotes Vap-, OSBP and Sac1-dependent pathogen vacuole remodeling
Description	The causative agent of Legionnaires’ disease, Legionella pneumophila, governs interactions with host cells by secreting ca. 330 different “effector” proteins. The facultative intracellular bacteria replicate in macrophages and amoeba within a unique compartment, the Legionella-containing vacuole (LCV). Hallmarks of LCV formation are the phosphoinositide (PI) lipid conversion from PtdIns(3)P to PtdIns(4)P, fusion with endoplasmic reticulum (ER)-derived vesicles and a tight association with the ER. Proteomics of purified LCVs revealed the presence of membrane contact sites (MCS) proteins implicated in lipid exchange. Using dually fluorescence-labeled Dictyostelium discoideum amoeba, we reveal that the VAMP-associated protein (Vap), the PtdIns(4)P 4-phosphatase Sac1, and the large fusion GTPase Sey1/atlastin-3 localize to the ER, but not to the LCV membrane, and these ER-resident proteins promote intracellular replication of L. pneumophila and LCV remodeling. Moreover, oxysterol binding proteins (OSBPs) exclusively localize to the ER (OsbH) or the LCV membrane (OsbK), respectively, and promote (OsbH) or restrict (OsbK) intracellular replication of L. pneumophila and LCV expansion. Furthermore, the PtdIns(4)P-subverting L. pneumophila effectors LepB and SidC also promote LCV remodeling. Taken together, the Legionella-driven PtdIns(4)P gradient at LCV-ER MCSs promotes Vap-, OSBP- and Sac1-dependent pathogen vacuole remodeling.
HostingRepository	PRIDE
AnnounceDate	2023-02-21
AnnouncementXML	Submission_2023-02-21_09:08:11.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SandraMaass
SpeciesList	scientific name: Legionella pneumophila subsp. pneumophila str. Philadelphia 1; NCBI TaxID: NCBITaxon:272624; scientific name: Dictyostelium discoideum; NCBI TaxID: NCBITaxon:44689;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-13 02:08:02	ID requested
⏵ 1	2023-02-21 09:08:12	announced
2	2023-11-14 07:28:35	announced	2023-11-14: Updated project metadata.

Vormittag S, H, ü, sler D, Haneburger I, Kroniger T, Anand A, Prantl M, Barisch C, Maa, ß S, Becher D, Letourneur F, Hilbi H, Legionella- and host-driven lipid flux at LCV-ER membrane contact sites promotes vacuole remodeling. EMBO Rep, 24(3):e56007(2023) [pubmed]

submitter keyword: large fusion GTPase, oxysterol binding protein, Legionella pneumophila, atlastin, Legionnaires’ disease, host-pathogen interaction, pathogen vacuole,Amoeba, Dictyostelium discoideum

DörteBecher
contact affiliation	Institute of Microbiology, University of Greifswald, Felix-Hausdorff-Strasse 8, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
SandraMaass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034490
     1. Label: PRIDE project
     2. Name: The Legionella-driven PtdIns(4)P gradient at LCV-ER membrane contact sites promotes Vap-, OSBP and Sac1-dependent pathogen vacuole remodeling