PXD034479-1

PXD034479 is an original dataset announced via ProteomeXchange.

Title	Perturbation of protein homeostasis brings plastids at the crossroad between repairment and dismantling
Description	The chloroplast proteome is a dynamic mosaic of plastid- and nuclear-encoded proteins, and plastid protein homeostasis is maintained through the balance between de novo protein synthesis and proteolysis. Intracellular communication pathways, including the pastid-to-nucleus retrograde communication, and the protein homeostasis machinery shape the chloroplast proteome based on developmental and physiological needs. However, the maintenance of fully functional chloroplasts is costly and under specific stress conditions the degradation of damaged chloroplasts is functional to the maintenance of a heathy population of photosynthesising organelles while promoting nutrient redistribution to sink tissues. In this study, we have addressed this complex regulatory chloroplast quality-control pathway by modulating the expression of two nuclear genes encoding the plastid ribosomal proteins PRPS1 and PRPL4. By transcriptomics, proteomics and transmission electron microscopy analyses, we show that the increased expression of PRPS1 gene leads to chloroplast degradation and early flowering, as an escaping strategy from stress conditions. On the contrary, the overaccumulation of PRPL4 protein is kept under control by the increased accumulation of plastid chaperones and other components of the unfolded protein response (cpUPR) regulatory mechanism. This study advances our understanding of the molecular mechanisms underlying chloroplast retrograde communication and provides new insight into cellular responses to impaired plastid protein homeostasis.
HostingRepository	PRIDE
AnnounceDate	2023-05-12
AnnouncementXML	Submission_2023-05-12_03:59:43.469.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MilenaMarsoni
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 08:25:51	ID requested
⏵ 1	2023-05-12 03:59:43	announced
2	2023-11-14 07:15:28	announced	2023-11-14: Updated project metadata.

submitter keyword: chloroplast retrograde communication,plastid protein homeostasis, the plastid ribosomal proteins

GuidoDomingo
contact affiliation	University of Insubria
contact email	g.domingo@uninsubria.it
lab head
MilenaMarsoni
contact affiliation	University of Insubria
contact email	milena.marsoni@uninsubria.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034479
     1. Label: PRIDE project
     2. Name: Perturbation of protein homeostasis brings plastids at the crossroad between repairment and dismantling