PXD034476-2

PXD034476 is an original dataset announced via ProteomeXchange.

Title	Deep Proteome profiling reveals signatures of age and sex differences in paw skin and sciatic nerve of naïve mice
Description	The age and sex of studied animals profoundly impacts experimental outcomes in animal-based biomedical research. However, most preclinical studies in mice use a wide-spanning age range from 4 to 14 weeks and do not assess study parameters in male and female mice in parallel. This raises concerns regarding reproducibility and neglect of potentially relevant age and sex differences. Furthermore, the molecular setup of tissues in dependence of age and sex is unknown in naïve mice precluding efficient translational research. Here, we first compared two different mass spectrometric acquisition methods – DDA- and DIA-PASEF – in order to maximize the depth of proteome quantitation. We then employed an optimized workflow of quantitative proteomics based on DIA-PASEF followed by DIA-NN data analysis, and revealed significant differences in mouse paw skin and sciatic nerve (SCN) when comparing (i) male and female mice, and, in parallel, (ii) adolescent mice (4 weeks) with adult mice (14 weeks).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:07.535.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Xian
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 08:24:16	ID requested
1	2022-11-29 03:49:59	announced
⏵ 2	2023-11-14 08:57:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: mouse, dia-pasef, skin, sex, sciatic nerve, proteome, age

Manuela Schmidt
contact affiliation	Systems Biology of Pain,Division of Pharmacology & Toxicology, Department of Pharmaceutical Sciences, University of Vienna
contact email	manuela_schmidt@univie.ac.at
lab head
Feng Xian
contact affiliation	Division of Pharmacology and Toxicology, Department of Pharmaceutical Sciences, University of Vienna
contact email	feng.xian@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034476
     1. Label: PRIDE project
     2. Name: Deep Proteome profiling reveals signatures of age and sex differences in paw skin and sciatic nerve of naïve mice