PXD034455 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Single locus phosphoproteomics reveals phosphorylation of RPA-1 is required for generation of single-strand DNA following a break at a subtelomeric locus |
Description | Damage to the genetic material of the cell poses a universal threat to all forms of life. Central to the DNA damage response (DDR) is a phosphorylation signalling cascade that leads to the co-ordination of the cellular response to a DNA break. Identifying the proteins that are phosphorylated is crucial to understanding the mechanisms underlying this DDR. We have used SILAC-based quantitative phosphoproteomics to profile changes in phosphorylation site abundance following a single double strand break (DSB) at a chromosome internal locus and the subtelomeric bloodstream form expression site in Trypanosoma brucei. We report >6500 phosphorylation sites, including a core set of 211 DSB responsive phosphorylation sites. Along with phosphorylation of canonical DNA damage factors, we find that there is a striking distinction between the proteins phosphorylated in response to a chromosome internal DSB and one at the active BES and describe a single phosphorylation event on Replication factor A (RPA) 1 that is required for efficient resection at a bloodstream form expression site |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-04 |
AnnouncementXML | Submission_2024-09-04_03:41:33.994.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MARIETTE MATONDO |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-10 02:33:18 | ID requested | |
⏵ 1 | 2024-09-04 03:41:34 | announced | |
2 | 2024-10-22 06:57:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Trypanosoma brucei,Phosphoproteomics, SILAC, DNA damage |
Contact List
Lucy Glover |
contact affiliation | Institut Pasteur, Université Paris Cité, Trypanosome Molecular Biology, Department of Parasites and Insect Vectors, F-75015, Paris, France |
contact email | lucy.glover@pasteur.fr |
lab head | |
MARIETTE MATONDO |
contact affiliation | Pasteur |
contact email | mariette.matondo@pasteur.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034455
- Label: PRIDE project
- Name: Single locus phosphoproteomics reveals phosphorylation of RPA-1 is required for generation of single-strand DNA following a break at a subtelomeric locus