PXD034454-1

PXD034454 is an original dataset announced via ProteomeXchange.

Title	bladder cancer patient urine exosomes
Description	Extracellular vesicles (EVs), particularly nano-sized small EV exosomes, are emerging biomarker sources. However, due to heterogeneous populations secreted from diverse cell types, mapping exosome multi-omic molecular information specifically to their pathogenesis origin for cancer biomarker identification is still extraordinarily challenging. Herein, we introduced a novel 3D-structured nanographene immunomagnetic particles (NanoPoms) with unique flower pom-poms morphology and photo-click chemistry for specific marker-defined capture and release of intact exosome. This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome. The NanoPoms prepared exosomes also exhibit distinctive in vivo biodistribution patterns, highlighting the highly viable and integral quality. The developed method is simple and straightforward, which is applicable to nearly all types of biological fluids and amenable for enrichment, scale up, and high-throughput exosome isolation.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:40:47.166.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034454
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	MEI HE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 02:32:58	ID requested
⏵ 1	2024-10-08 11:40:48	announced

He N, Thippabhotla S, Zhong C, Greenberg Z, Xu L, Pessetto Z, Godwin AK, Zeng Y, He M, Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection. Commun Biol, 5(1):660(2022) [pubmed]

10.6019/PXD034454;

10.1038/s42003-022-03598-0;

submitter keyword: exosomes, urine, bladder cancer,human

Mei He
contact affiliation	University of FLorida
contact email	mhe@cop.ufl.edu
lab head
MEI HE
contact affiliation	University of Florida
contact email	mhe@cop.ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD034454

PRIDE project URI

• PRIDE
  1. PXD034454
     1. Label: PRIDE project
     2. Name: bladder cancer patient urine exosomes