PXD034454 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | bladder cancer patient urine exosomes |
Description | Extracellular vesicles (EVs), particularly nano-sized small EV exosomes, are emerging biomarker sources. However, due to heterogeneous populations secreted from diverse cell types, mapping exosome multi-omic molecular information specifically to their pathogenesis origin for cancer biomarker identification is still extraordinarily challenging. Herein, we introduced a novel 3D-structured nanographene immunomagnetic particles (NanoPoms) with unique flower pom-poms morphology and photo-click chemistry for specific marker-defined capture and release of intact exosome. This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome. The NanoPoms prepared exosomes also exhibit distinctive in vivo biodistribution patterns, highlighting the highly viable and integral quality. The developed method is simple and straightforward, which is applicable to nearly all types of biological fluids and amenable for enrichment, scale up, and high-throughput exosome isolation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:40:47.166.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034454 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | MEI HE |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-10 02:32:58 | ID requested | |
⏵ 1 | 2024-10-08 11:40:48 | announced | |
Publication List
He N, Thippabhotla S, Zhong C, Greenberg Z, Xu L, Pessetto Z, Godwin AK, Zeng Y, He M, Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection. Commun Biol, 5(1):660(2022) [pubmed] |
10.6019/PXD034454; |
10.1038/s42003-022-03598-0; |
Keyword List
submitter keyword: exosomes, urine, bladder cancer,human |
Contact List
Mei He |
contact affiliation | University of FLorida |
contact email | mhe@cop.ufl.edu |
lab head | |
MEI HE |
contact affiliation | University of Florida |
contact email | mhe@cop.ufl.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034454
- Label: PRIDE project
- Name: bladder cancer patient urine exosomes