PXD034417-1

PXD034417 is an original dataset announced via ProteomeXchange.

Title	Engineering consortia by polymeric microbial swarmbots
Description	Synthetic microbial consortia represent a new frontier for synthetic biology given that they can solve more complex problems than monocultures. However, most attempts to co-cultivate these artificial communities fail because of the ‘‘winner-takes-all’’ in nutrients competition. In soil, multiple species can coexist with a spatial organization. Inspired by nature, here we show that an engineered spatial segregation method can assemble stable consortia with both flexibility and precision. We create microbial swarmbot consortia (MSBC) by encapsulating subpopulations with polymeric microcapsules. The crosslinked structure of microcapsules fences microbes, but allows the transport of small molecules and proteins. MSBC method enables the assembly of various synthetic communities and the precise control over the subpopulations. These capabilities can readily modulate the division of labor and communication. Our work integrates the synthetic biology and material science to offer new insights into consortia assembly and server as foundation to diverse applications from biomanufacturing to engineered photosynthesis.
HostingRepository	PRIDE
AnnounceDate	2022-07-04
AnnouncementXML	Submission_2022-07-04_00:40:01.523.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xi zhang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 13:12:16	ID requested
⏵ 1	2022-07-04 00:40:01	announced
2	2023-11-14 08:56:35	announced	2023-11-14: Updated project metadata.

10.1038/S41467-022-31467-1;

submitter keyword: synthetic microbial consortia

spatial segregation

polymeric microcapsules

division of labor

communication

Zhuojun Dai
contact affiliation	Shenzhen Institute of Advanced Technology (SIAT), Chinese Academy of Sciences
contact email	zj.dai@siat.ac.cn
lab head
Xi zhang
contact affiliation	Shenzhen Institutes of Advanced Technology (SIAT) of the Chinese Academy of Science (CAS)
contact email	xi.zhang1@siat.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034417

PRIDE project URI

• PRIDE
  1. PXD034417
     1. Label: PRIDE project
     2. Name: Engineering consortia by polymeric microbial swarmbots