PXD034413 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatially-targeted optical microproteomics timecourse of rat myocardial infarction |
Description | Myocardial infarction (MI) results from occlusion of blood supply to the heart muscle causing death of cardiac muscle cells. Following myocardial infarction (MI), scar formation acts to mechanically stabilise the injured heart to prevent rupture and death. While fibroblasts and macrophages are implicated in post-MI scar formation and maturation, their exact contributions to this process are poorly characterised, especially in the long-term (i.e., beyond 1-week post-MI). Here, we employ state-of-the-art spatially-targetted optical micro-proteomics (STOMP) to isolate proteomes of tissue fractions enriched for fibroblasts (sma+) and macrophages (cd68+) over a 6-week post-MI timecourse and compare them to whole-scar proteome. We illustrate dynamic, specific changes in sma- and cd68-enriched fraction protein composition over 1-6 weeks post-MI with some of these changes reflected in whole-infarct preparations. These results link specific cell populations to specific protein changes in whole infarct. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-31 |
AnnouncementXML | Submission_2023-10-31_10:31:58.378.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | VenkateshMallikarjun |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | dehydroalanine (Cys); acetylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); iodoacetic acid derivatized residue; deamidated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-09 08:34:59 | ID requested | |
⏵ 1 | 2023-10-31 10:31:58 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cardiac, heart, macrophage, cd68,rat, sma, microproteomics, fibroblast, myocardial infarction |
Contact List
SarahEwald |
contact affiliation | Assistant Professor of Microbiology, Immunology, and Cancer Biology Associate Director for Diversity, Equity, and Inclusio at the Carter Immunology Center University of Virginia Charlottesville, VA |
contact email | e2s@virginia.edu |
lab head | |
VenkateshMallikarjun |
contact affiliation | University of Manchester |
contact email | vm9uq@virginia.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034413
- Label: PRIDE project
- Name: Spatially-targeted optical microproteomics timecourse of rat myocardial infarction