PXD034406-1

PXD034406 is an original dataset announced via ProteomeXchange.

Title	Retrospective longitudinal monitoring of multiple myeloma patients by mass spectrometry using archived serum protein electrophoresis gels and de novo sequence analysis
Description	M-proteins are antibody molecules produced by a clone of Multiple Myeloma cells. Thus, they are used as a disease activity marker. Traditionally, serum protein electrophoresis gels (SPEP) are used to quantify M-proteins. While effective for diagnostics, sensitivity of SPEP is limited. Here, we demonstrate that M-protein gels can be excised from SPEP gels, even when the band is undetectable by visible inspection. Clonotypic peptides are identified by de novo sequencing of mass spectrometry data, and these clonotypic peptides are then monitored in the longitudinal data covering the course of disease. Thus, it is possible to follow disease activity in a blood sample with a sensitivity that may be similar to that of minimal residual diseas platforms that rely on bone marrow biopsies.
HostingRepository	PanoramaPublic
AnnounceDate	2022-08-10
AnnouncementXML	Submission_2022-08-10_07:57:57.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martijn van Duijn
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-08 10:18:54	ID requested
⏵ 1	2022-08-10 07:57:58	announced

Noori S, Zajec M, Russcher H, Tintu AN, Broijl A, Jacobs JFM, Luider TM, de Rijke YB, vanDuijn MM, Retrospective Longitudinal Monitoring of Multiple Myeloma Patients by Mass Spectrometry Using Archived Serum Protein Electrophoresis Gels and De Novo Sequence Analysis. Hemasphere, 6(8):e758(2022) [pubmed]

submitter keyword: Minimal Residual Disease, Serum protein Electrophoresis, Mass Spectrometry, Multiple Myeloma

Martijn van Duijn
contact affiliation	Erasmus MC
contact email	m.m.vanduijn@erasmusmc.nl
lab head
Martijn van Duijn
contact affiliation	Erasmus MC
contact email	m.m.vanduijn@erasmusmc.nl
dataset submitter

Panorama Public dataset URI