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PXD034406 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRetrospective longitudinal monitoring of multiple myeloma patients by mass spectrometry using archived serum protein electrophoresis gels and de novo sequence analysis
DescriptionM-proteins are antibody molecules produced by a clone of Multiple Myeloma cells. Thus, they are used as a disease activity marker. Traditionally, serum protein electrophoresis gels (SPEP) are used to quantify M-proteins. While effective for diagnostics, sensitivity of SPEP is limited. Here, we demonstrate that M-protein gels can be excised from SPEP gels, even when the band is undetectable by visible inspection. Clonotypic peptides are identified by de novo sequencing of mass spectrometry data, and these clonotypic peptides are then monitored in the longitudinal data covering the course of disease. Thus, it is possible to follow disease activity in a blood sample with a sensitivity that may be similar to that of minimal residual diseas platforms that rely on bone marrow biopsies.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMartijn van Duijn
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-08 10:18:54ID requested
12022-08-10 07:57:58announced
Publication List
Noori S, Zajec M, Russcher H, Tintu AN, Broijl A, Jacobs JFM, Luider TM, de Rijke YB, vanDuijn MM, Retrospective Longitudinal Monitoring of Multiple Myeloma Patients by Mass Spectrometry Using Archived Serum Protein Electrophoresis Gels and De Novo Sequence Analysis. Hemasphere, 6(8):e758(2022) [pubmed]
Keyword List
submitter keyword: Minimal Residual Disease, Serum protein Electrophoresis, Mass Spectrometry, Multiple Myeloma
Contact List
Martijn van Duijn
contact affiliationErasmus MC
contact emailm.m.vanduijn@erasmusmc.nl
lab head
Martijn van Duijn
contact affiliationErasmus MC
contact emailm.m.vanduijn@erasmusmc.nl
dataset submitter
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