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PXD034373-1

PXD034373 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of bile acid-interacting proteins in Salmonella Typhimurium (14028S) by chemoproteomics
DescriptionThe microbiota generates structurally diverse small molecules that can regulate host physiology and disease. Of these microbiota metabolites, bile acids have emerged as important modulators of host immunity and microbial pathogenesis. While the modes of action for different bile acids on host pathways are becoming more apparent, the mechanisms by which these prominent microbiota metabolites suppress microbial virulence pathways are less clear. To identify the direct protein targets of bile acids in Salmonella, we have generated three photoaffinity bile acid reporters (alk-X-CDCA, alk-X-UDCA, alk-X-LCA) and performed chemical proteomics. Using a combination of photocrosslinking with bile acid chemical reporters and label-free proteomics, we performed a quantitative analysis of bile acid interacting proteins in Salmonella with or without UV-mediated photocrosslinking. These studies revealed bile acid can interact with many Salmonella proteins, such as extracellular or secreted proteins, T3SS components and motility-related proteins, as predicted by Gene Ontology analysis. In addition, cytoplasmic and membrane proteins including metabolic enzymes are also identified. Notably, HilD, an important transcriptional regulator of S. Typhimurium virulence was also identified by the alk-X-CDCA reporter. This study highlights the utility of chemical proteomics to identify the direct protein targets and mechanisms of action for microbiota metabolites in bacterial pathogens.
HostingRepositoryPRIDE
AnnounceDate2022-09-29
AnnouncementXMLSubmission_2022-09-28_19:50:47.872.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXinglin Yang
SpeciesList scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028; NCBI TaxID: 1299114;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-06 06:40:33ID requested
12022-09-28 19:50:48announced
22023-11-14 08:56:18announced2023-11-14: Updated project metadata.
Publication List
10.1038/S41589-022-01122-3;
Keyword List
submitter keyword: Bile acids, Salmonella, chemoproteomics, photoaffinity
Contact List
Howard C. Hang
contact affiliationDepartments of Immunology & Microbiology and Chemistry Scripps Research
contact emailhhang@scripps.edu
lab head
Xinglin Yang
contact affiliationScripps Research
contact emailxiyang@scripps.edu
dataset submitter
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Dataset FTP location
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