PXD034329-2

PXD034329 is an original dataset announced via ProteomeXchange.

Title	Pulldown of proteins interacting with SGOL1-AS1 in AML
Description	Epigenetic dysregulation is a common feature of acute myeloid leukemia (AML). Recently it has become clear that long noncoding RNAs (lncRNAs) can play a key role in epigenetic regulation, and consequently also dysregulation. Currently, our understanding of the requirements and roles of lncRNAs in AML is still limited. Using CRISPRi screening, we identified the lncRNA SGOL1-AS1 as an essential regulator of survival in THP-1 AML cells. We use RNA affinity purification using a biotinylated bait to pull down binding partners of the lncRNA, SGOL1-AS1. The identified proteins show a signficant enrichment for chromatin-modifying proteins involved in gene repression and chromosome organization.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:45.672.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034329
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anna Hoyle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-06 01:08:41	ID requested
1	2023-06-12 04:03:07	announced
⏵ 2	2023-11-14 09:00:46	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:47:45	announced	2024-10-22: Updated project metadata.

Selkirk E, Patel R, Hoyle A, Lie-A-Ling M, Smith D, Swift J, Lacaud G, SGOL1-AS1 enhances cell survival in acute myeloid leukemia by maintaining pro-inflammatory signaling. Heliyon, 8(11):e11362(2022) [pubmed]

10.6019/PXD034329;

submitter keyword: long non-coding RNA,lncRNA, AML, Acute Myeloid Leukemia

Georges Lacaud
contact affiliation	Stem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, Manchester, UK.
contact email	Georges.Lacaud@cruk.manchester.ac.uk
lab head
Anna Hoyle
contact affiliation	University of Manchester
contact email	anna.hoyle@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD034329

PRIDE project URI

• PRIDE
  1. PXD034329
     1. Label: PRIDE project
     2. Name: Pulldown of proteins interacting with SGOL1-AS1 in AML