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PXD034329

PXD034329 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePulldown of proteins interacting with SGOL1-AS1 in AML
DescriptionEpigenetic dysregulation is a common feature of acute myeloid leukemia (AML). Recently it has become clear that long noncoding RNAs (lncRNAs) can play a key role in epigenetic regulation, and consequently also dysregulation. Currently, our understanding of the requirements and roles of lncRNAs in AML is still limited. Using CRISPRi screening, we identified the lncRNA SGOL1-AS1 as an essential regulator of survival in THP-1 AML cells. We use RNA affinity purification using a biotinylated bait to pull down binding partners of the lncRNA, SGOL1-AS1. The identified proteins show a signficant enrichment for chromatin-modifying proteins involved in gene repression and chromosome organization.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:00:45.672.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD034329
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAnna Hoyle
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-06 01:08:41ID requested
12023-06-12 04:03:07announced
22023-11-14 09:00:46announced2023-11-14: Updated project metadata.
Publication List
Selkirk E, Patel R, Hoyle A, Lie-A-Ling M, Smith D, Swift J, Lacaud G, SGOL1-AS1 enhances cell survival in acute myeloid leukemia by maintaining pro-inflammatory signaling. Heliyon, 8(11):e11362(2022) [pubmed]
10.6019/PXD034329;
Keyword List
submitter keyword: long non-coding RNA,lncRNA, AML, Acute Myeloid Leukemia
Contact List
Georges Lacaud
contact affiliationStem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, Manchester, UK.
contact emailGeorges.Lacaud@cruk.manchester.ac.uk
lab head
Anna Hoyle
contact affiliationUniversity of Manchester
contact emailanna.hoyle@postgrad.manchester.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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