PXD034267-2

PXD034267 is an original dataset announced via ProteomeXchange.

Title	Data-Independent Acquisition Proteomics Reveals Circulating Biomarkers of Coronary Chronic Total Occlusion in Humans
Description	The pathophysiology of chronic coronary total occlusion (CTO) has not been fully elucidated. In the present study, we aimed to investigate the potential plasma biomarkers associated with the pathophysiologic progression of CTO and identify protein dynamics in the plasma of CTO vessels immediately after successful revascularization. We quantitatively analyzed the plasma proteome profiles of controls (CON, n=10) and patients with CTO pre- and post- percutaneous coronary intervention (PCI) (CTO, n=10) by data-independent acquisition proteomics. A total of 1936 proteins with 69 differentially expressed proteins (DEPs) were detected in the plasma of patients with CTO through quantitative proteomics analysis. For all these DEPs, gene ontology (GO) analysis and protein-protein interaction (PPI) analysis were performed. The results showed that most of the proteins were related to the negative regulation of proteolysis, regulation of peptidase activity, negative regulation of hydrolase activity, humoral immune response, and lipid location. Furthermore, we identified 1927 proteins with 43 DEPs in the plasma of patients with CTO vessels after immediately successful revascularization compared to pre-PCI. GO analysis revealed that the above DEPs were enriched in the biological processes of extracellular structure organization, protein activation cascade, negative regulation of response to external stimulus, plasminogen activation, and fibrinolysis. More importantly, seven proteins, ADH4, CSF1, galectin, LPL, IGF2, IgH, and LGALS1, were found to be dynamically altered in plasma during the pathophysiological progression of CTO vessels and following successful revascularization.
HostingRepository	iProX
AnnounceDate	2022-06-02
AnnouncementXML	Submission_2023-01-28_18:21:23.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Zhou
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-02 01:06:11	ID requested
1	2022-12-04 16:26:53	announced
⏵ 2	2023-01-28 18:21:24	announced	2023-01-29: Update publication information.

Li J, Jiang XJ, Wang QH, Wu XL, Qu Z, Song T, Wan WG, Zheng XX, Yi X, Data-independent acquisition proteomics reveals circulating biomarkers of coronary chronic total occlusion in humans. Front Cardiovasc Med, 9():960105(2022) [pubmed]

submitter keyword: data-independent acquisition proteomics, coronary chronic total occlusion, protein dynamics, percutaneous coronary intervention, human

Xin Yi
contact affiliation	Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China
contact email	yixin321624@126.com
lab head
Qian Zhou
contact affiliation	Shanghai Omicsspace Biotech Co., Ltd.
contact email	zhouqian@omicsspace.com
dataset submitter

iProX dataset URI