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PXD034256-1

PXD034256 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFASII antibiotic adaptation reprograms Staphylococcus aureus behavior
DescriptionProving functionality in the host environment is a crucial step in antimicrobial development pipelines. Antibiotics targeting fatty acid synthesis (FASII) of the major pathogen Staphylococcus aureus actively inhibit FASII but do not prevent in vivo growth, as bacteria compensate the FASII block by using environmental fatty acids. We used proteomics and phosphoproteomics to elucidate S. aureus responses to anti-FASII in host-relevant conditions. S. aureus responded to anti-FASII treatment in serum by massive reprogramming. A striking inverse correlation was observed in anti-FASII-adapted S. aureus, between amounts of stress response proteins that increase, and virulence factors that decrease. These findings suggest that anti-FASII adapted cells might be better prepared for survival and less equipped to damage the host. Infection by anti-FASII-adapted versus non-treated S. aureus was challenged in the Galleria mellonella model. Time to mortality was longer in insects infected by anti-FASII-treated bacteria compared to those infected by non-treated S. aureus. However, bacterial counts in infected dead insects were comparable for both groups. These results support the hypothesis that higher stress response and lower virulence factor expression, as shown here in FASII-antibiotic-adapted bacteria, may set the stage for persistent infection
HostingRepositoryPRIDE
AnnounceDate2023-10-09
AnnouncementXMLSubmission_2023-10-09_09:35:15.982.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCélineHenry
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-01 11:21:03ID requested
12023-10-09 09:35:16announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: FASII antibiotic adaptation reprograms Staphylococcus aureus behavior
Contact List
AlexandraGruss
contact affiliationUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
contact emailalexandra.gruss@inrae.fr
lab head
CélineHenry
contact affiliationINRAE
contact emailceline.henry@inrae.fr
dataset submitter
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Dataset FTP location
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