PXD034242-1

PXD034242 is an original dataset announced via ProteomeXchange.

Title	The non-classical major-histocompatibility-complex II protein SLA-DM is crucial for African swine fever virus replication
Description	African swine fever virus (ASFV) is a lethal animal pathogen which enters its host cells through endocytosis. So far, host factors specifically required for ASFV replication have been barely identified. In this study a genome-wide CRISPR/Cas9 knockout screen in porcine cells indicated that the genes RFXANK, RFXAP, SLA-DMA, SLA-DMB, and CIITA are important for productive ASFV infection. The proteins encoded by these genes belong to the major-histocompatibility-complex II (MHC II), or swine-leucocyte-antigen-complex II (SLA II). RFXAP and CIITA are MHC II-specific transcription factors, whereas SLA-DMA/B are subunits of the non-classical MHC II molecule SLA-DM. Targeted knockout of either of these genes led to severe replication defects of different ASFV isolates, reflected by substantially reduced plating efficiency, cell-to-cell spread, and progeny virus titers. For the characterization of the knockouts on a proteome level the protein contents of the knockout cell lines were analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-08-21
AnnouncementXML	Submission_2023-08-21_06:27:52.077.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AxelKarger
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-01 05:00:30	ID requested
⏵ 1	2023-08-21 06:27:52	announced
2	2023-11-14 07:08:29	announced	2023-11-14: Updated project metadata.

submitter keyword: SLA-DMB, African swine fever virus (ASFV), Major histocompatibility complex II (MHC II), SLA-DMA,Genome-wide CRISPR/Cas9 knockout screening (GeCKo), Swine leucocyte antigen complex II (SLA II)

AxelKarger
contact affiliation	Institute of molecular virology and cell biology
contact email	axel.karger@fli.de
lab head
AxelKarger
contact affiliation	Friedrich-Loeffler-Institut
contact email	axel.karger@fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034242
     1. Label: PRIDE project
     2. Name: The non-classical major-histocompatibility-complex II protein SLA-DM is crucial for African swine fever virus replication