PXD034156-1

PXD034156 is an original dataset announced via ProteomeXchange.

Title	DYRK1A regulates B cell class switch recombination through phosphorylation of MSH6
Description	Effective protection from viral infections depends on the antibody-specific isotype. Here, we found that the protein kinase, DYRK1A, is essential for B cell-mediated protection from viral infection and vaccination through regulation of class switch recombination (CSR). Dyrk1a-deficient B cells were impaired in CSR activity in vivo and in vitro. Phospho-proteomic screens and kinase-activity assays identified MSH6, a DNA mismatch repair protein, as a direct substrate for DYRK1A, and deletion of a single phosphorylation site attenuated CSR. After CSR and germinal center seeding, DYRK1A was required for proper clonal expansion of antigen-specific B cells through attenuation of proliferation. These findings reveal DYRK1A-mediated biological mechanisms of antibody-mediated immune responses that may be used for manipulation in antibody-mediated autoimmunity
HostingRepository	PRIDE
AnnounceDate	2023-03-16
AnnouncementXML	Submission_2023-03-16_14:59:13.610.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MeitalKupervaser
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-27 06:21:04	ID requested
⏵ 1	2023-03-16 14:59:13	announced
2	2023-11-14 08:59:34	announced	2023-11-14: Updated project metadata.

submitter keyword: antibody, clonal selection, B cell, class-switch recombination,Humoral immunity

ZivShulman
contact affiliation	Department of Immunology, Weizmann Institute of Science, Rehovot, Israel
contact email	ziv.shulman@weizmann.ac.il
lab head
MeitalKupervaser
contact affiliation	Weizmann Institute of Science
contact email	meital.kupervaser@weizmann.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034156
     1. Label: PRIDE project
     2. Name: DYRK1A regulates B cell class switch recombination through phosphorylation of MSH6