PXD034135 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-Cov-2 innate immune evasion |
Description | We used two model systems to characterise the changes caused by SARS-CoV-2 using quantitative proteomics. We used primary human epithelial cells differentiated at the air-liquid interface, first describing the relative abundance of proteins found in each major cell type of the epithelium, and then characterised the infection of ciliated cells specifically as these showed the greatest susceptibility to SARS-CoV-2 infection. We also used a lung epithelial cell line, CaLu-3, and compared the infection with B.29 and B.1.1.7 (Alpha) variant over a time-course of infection. In each case, immunostaining for nucleoprotein combined with FACS allowed for the purification of infected cells from uninfected ‘bystander’ cells. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-26 |
AnnouncementXML | Submission_2022-05-26_07:43:45.925.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034135 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | James Williamson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-26 01:18:07 | ID requested | |
⏵ 1 | 2022-05-26 07:43:46 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SARS-Cov-2, HBEC, |
Contact List
Paul Lehner |
contact affiliation | Paul J Lehner PhD FRCP FMed Sci Professor of Immunology and Medicine Wellcome Trust Principal Research Fellow Honorary Consultant Infectious Diseases Cambridge Institute of Therapeutic Immunology and Infectious Disease Jeffrey Cheah Biomedical Centre Cambridge Biomedical Campus Puddicombe Way Cambridge CB2 OAW |
contact email | pjl30@cam.ac.uk |
lab head | |
James Williamson |
contact affiliation | University of Cambridge |
contact email | jcw76@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034135
- Label: PRIDE project
- Name: Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-Cov-2 innate immune evasion