PXD034132-1

PXD034132 is an original dataset announced via ProteomeXchange.

Title	New nanoparticle Venetin-1 from the coelomic fluid of the earthworm Dendrobaena veneta as a promising agent for the treatment of non-small cell lung cancer A549
Description	The present research shows the antitumor activity of a protein-polysaccharide complex Venetin-1 obtained from the fluid of Dendrobaena veneta earthworms against A549 cancer cells. The investigations are a continuation of experiments on the antitumor activity of the coelomic fluid from this species. The Venetin-1 nanoparticle was obtained after thermal treatment of the coelomic fluid, separation from coelomocytes, filtration, and lyophilization. The preparation showed a selective effect on cancer cells while sparing normal cells. Venetin-1 was effective against the lung cancer cells at doses of 31.3 and 62.5 µg/ml, which was imaged using light microscopy and scanning electron microscopy (SEM). The cells died mainly via the apoptosis pathway. Necrotic cells appeared sporadically in the microscopic view. SEM imaging revealed complete destruction of the A549 cells after the incubation with Venetin-1. The atomic force microscopy (AFM) analyses showed changes in the topography, peak force error images, and Young’s modulus (elasticity) of the A549 cells after the incubation with Venetin-1. The transmission electron cryomicroscopy (Cryo-TEM) microscopic analysis indicated a polymeric nature of the analyzed preparation. The samples of Venetin-1 showed a very homogeneous size profile with the microparticle size determined to be around 58.23 nm. A significant decrease in Venetin-1 binding to sphingomyelin was observed. Venetin-1 lost its pore-forming activity or deactivation of the pore-forming activity occurred. This confirms the lack of hemolytic capacity of Venetin-1 towards red blood cells.
HostingRepository	PRIDE
AnnounceDate	2022-10-07
AnnouncementXML	Submission_2022-10-07_05:13:51.352.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Czaplewska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-26 01:16:59	ID requested
⏵ 1	2022-10-07 05:13:51	announced
2	2023-11-14 08:56:50	announced	2023-11-14: Updated project metadata.

10.1038/S41598-022-21665-8;

submitter keyword: A549, Dendrobaena Veneta, SPR, cancer

Paulina Czaplewska
contact affiliation	Universuty of Gdansk, Intercollegiate Faculty of Biotechnology, Laboratory of Mass Spectrometry
contact email	paulina.czaplewska@ug.edu.pl
lab head
Paulina Czaplewska
contact affiliation	University of Gdansk
contact email	paulina.czaplewska@ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034132

PRIDE project URI

• PRIDE
  1. PXD034132
     1. Label: PRIDE project
     2. Name: New nanoparticle Venetin-1 from the coelomic fluid of the earthworm Dendrobaena veneta as a promising agent for the treatment of non-small cell lung cancer A549