<<< Full experiment listing

PXD034121-1

PXD034121 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic response of A549 lung cancer cell line to Venetin-1
DescriptionEarthworm's celomic fluid has long attracted scientists' interest due to its toxic properties. It has been shown that subjecting it to a short heating at 70 ° C, which effectively reduces the toxicity to healthy cells, and demonstrates the selective action of the obtained Venetin-1 preparation against Candida albicans cells, as well as tumor cells from the A549 line. In order to find out about the molecular mechanisms behind the anti-cancer properties of the preparation, the conducted research investigated the proteome response of A549 cells to the presence of Venetin-1. The sequential window acquisition of all theoretical mass spectra (SWATH-MS) methodology was used for the analysis, which allows for a relative quantitative analysis to be carried out without radiolabelling. The results showed that the formulation did not induce significant proteome responses in healthy BEAS cells. In the case of the tumor line, 31 proteins were up-regulated and 18 proteins down-regulated. Proteins with increased expression in neoplastic cells are mainly associated with the mitochondrion, membrane transport and the endoplasmic reticulum. In the case of altered proteins, Venetin-1 interferes in proteins that stabilize the structures, i.e. keratin, glycolysis / gluconeogenesis and metabolic processes. This information shows the potential of Venetin-1, which may be used in the treatment of lung cancer in the future.
HostingRepositoryPRIDE
AnnounceDate2023-06-02
AnnouncementXMLSubmission_2023-06-02_07:40:23.428.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaulinaCzaplewska
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-25 06:55:27ID requested
12023-06-02 07:40:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, SWATH,Venetin-1, earthworms, coelomic fluid
Contact List
PaulinaCzaplewska
contact affiliationUniversity of Gdansk, Laboratory of Mass Spectrometry
contact emailpaulina.czaplewska@ug.edu.pl
lab head
PaulinaCzaplewska
contact affiliationUniversity of Gdansk
contact emailpczaplewska@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD034121
PRIDE project URI
Repository Record List
[ + ]