PXD034121-1

PXD034121 is an original dataset announced via ProteomeXchange.

Title	Proteomic response of A549 lung cancer cell line to Venetin-1
Description	Earthworm's celomic fluid has long attracted scientists' interest due to its toxic properties. It has been shown that subjecting it to a short heating at 70 ° C, which effectively reduces the toxicity to healthy cells, and demonstrates the selective action of the obtained Venetin-1 preparation against Candida albicans cells, as well as tumor cells from the A549 line. In order to find out about the molecular mechanisms behind the anti-cancer properties of the preparation, the conducted research investigated the proteome response of A549 cells to the presence of Venetin-1. The sequential window acquisition of all theoretical mass spectra (SWATH-MS) methodology was used for the analysis, which allows for a relative quantitative analysis to be carried out without radiolabelling. The results showed that the formulation did not induce significant proteome responses in healthy BEAS cells. In the case of the tumor line, 31 proteins were up-regulated and 18 proteins down-regulated. Proteins with increased expression in neoplastic cells are mainly associated with the mitochondrion, membrane transport and the endoplasmic reticulum. In the case of altered proteins, Venetin-1 interferes in proteins that stabilize the structures, i.e. keratin, glycolysis / gluconeogenesis and metabolic processes. This information shows the potential of Venetin-1, which may be used in the treatment of lung cancer in the future.
HostingRepository	PRIDE
AnnounceDate	2023-06-02
AnnouncementXML	Submission_2023-06-02_07:40:23.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PaulinaCzaplewska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 06:55:27	ID requested
⏵ 1	2023-06-02 07:40:23	announced

Dataset with its publication pending

submitter keyword: proteomics, SWATH,Venetin-1, earthworms, coelomic fluid

PaulinaCzaplewska
contact affiliation	University of Gdansk, Laboratory of Mass Spectrometry
contact email	paulina.czaplewska@ug.edu.pl
lab head
PaulinaCzaplewska
contact affiliation	University of Gdansk
contact email	pczaplewska@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD034121

PRIDE project URI

• PRIDE
  1. PXD034121
     1. Label: PRIDE project
     2. Name: Proteomic response of A549 lung cancer cell line to Venetin-1