PXD034118 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Combining redox proteomics with proximity labeling to monitor localized cysteine oxidation |
Description | Reactive oxygen species (ROS) can modulate protein function through cysteine oxidation. Identifying targets of ROS can provide insight into uncharacterized ROS-regulated pathways. Several redox-proteomic workflows, such as OxICAT, exist to identify sites of cysteine oxidation. However, determining ROS targets localized within subcellular compartments and ROS hotspots remains challenging with existing workflows. Here, we present a chemoproteomic platform, PL-OxICAT, which combines proximity labeling (PL) with OxICAT to monitor localized cysteine oxidation events. We show that TurboID-based PL-OxICAT can monitor cysteine oxidation events within subcellular compartments such as the mitochondrial matrix and intermembrane space. Furthermore, we use APEX-based PL-OxICAT to monitor oxidation events within ROS hotspots by using endogenous ROS as the source of peroxide for APEX activation. Together, these platforms further hone our ability to monitor cysteine oxidation events within specific subcellular locations and ROS hotspots and provide a deeper understanding of the protein targets of endogenous and exogenous ROS. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-21 |
AnnouncementXML | Submission_2024-05-21_09:34:16.272.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eleni Kisty |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-25 06:54:15 | ID requested | |
⏵ 1 | 2024-05-21 09:34:17 | announced | |
Publication List
Kisty EA, Falco JA, Weerapana E, Redox proteomics combined with proximity labeling enables monitoring of localized cysteine oxidation in cells. Cell Chem Biol, 30(3):321-336.e6(2023) [pubmed] |
10.1016/j.chembiol.2023.02.006; |
Keyword List
submitter keyword: Cysteine oxidation, APEX, reactive oxygen species, OxICAT, TurboID, proximity labeling |
Contact List
Eranthie Weerapana |
contact affiliation | Chemistry Department, Boston College, USA |
contact email | eranthie@bc.edu |
lab head | |
Eleni Kisty |
contact affiliation | Boston College |
contact email | kisty@bc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034118
- Label: PRIDE project
- Name: Combining redox proteomics with proximity labeling to monitor localized cysteine oxidation