PXD034116-1

PXD034116 is an original dataset announced via ProteomeXchange.

Title	Site specific pseudouridine (Psi) on Leishmania rRNA regulates translation of subset of proteins
Description	Leishmania are unicellular parasites that cycle between the insect and mammalian host and is the causative agent of Leishmaniasis. Previous studies from our lab suggested that individual rRNA modification such as pseudouridine (Psi) and 2-O-methylation are developmentally regulated during the complex life cycle of these parasites. Ablation of a single snoRNA guiding Psi in the inter-subunit bridge, helix69 of the ribosome affects its affinity to translate specific mRNA. Mutational analysis in the pseudouridylation pocket of this snoRNA supports the notion that the guided modification and not the chaperone activity, is essential for the observed phenotype. Structural studies using CryoEM suggested that a single Psi modification can affect the rRNA structure. We propose that alteration in rRNA modifications could generate ribosomes preferentially translating state-beneficial proteins.
HostingRepository	PRIDE
AnnounceDate	2024-05-09
AnnouncementXML	Submission_2024-05-09_02:01:42.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tirza Doniger
SpeciesList	scientific name: Leishmania major strain Friedlin; NCBI TaxID: 347515;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 06:53:06	ID requested
⏵ 1	2024-05-09 02:01:42	announced
2	2024-10-22 06:38:40	announced	2024-10-22: Updated project metadata.

10.1016/J.CELREP.2024.114203;

submitter keyword: pseudouridine,Leishmania, ribosome, snoRNA, cryoEM

Prof. Shulamit Michaeli
contact affiliation	Bar Ilan University Faculty of Life Sciences
contact email	Shulamit.Michaeli@biu.ac.il
lab head
Tirza Doniger
contact affiliation	Bar-Ilan University
contact email	tirza.doniger@biu.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD034116

PRIDE project URI

• PRIDE
  1. PXD034116
     1. Label: PRIDE project
     2. Name: Site specific pseudouridine (Psi) on Leishmania rRNA regulates translation of subset of proteins