PXD034105-1

PXD034105 is an original dataset announced via ProteomeXchange.

Title	Characterising early phosphorylation events upon HER2 protein overexpression
Description	HER2 transduced cells which we will refer to as HER2-DOX –. These cells are 99% GFP positive (i.e., 99% cells have HER2 transduced, un-induced). As a control, we had GFP empty vector transduced MCF10A cells (95% have GFP transduced). Both cell types in triplicates. We had 4 time-points 0h, 30 mins, 4hours, and 7 hours (time duration for which HER2 will be induced). DOX was added to the GFP-MCF10A cells as a control. Only 1ug/ml of DOX was be used.
HostingRepository	PRIDE
AnnounceDate	2023-01-23
AnnouncementXML	Submission_2023-01-23_07:58:23.305.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034105
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	VinothiniRajeeve
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 03:19:54	ID requested
⏵ 1	2023-01-23 07:58:23	announced

10.6019/PXD034105;

submitter keyword: DOX,HER2, Labell-fee, phosphopoproteomics, GFP, MCF10A, LC-MS/MS

Professor PedroCutillas
contact affiliation	Centre for Genomics and Computational Biology Barts Cancer Institute Queen Mary, University of London John Vane Science Centre, Charterhouse Square, London EC1M 6BQ Tel: +44 (0)20 7882 8266
contact email	p.cutillas@qmul.ac.uk
lab head
VinothiniRajeeve
contact affiliation	Centre for Haemato Oncology
contact email	v.rajeeve@qmul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD034105

PRIDE project URI

• PRIDE
  1. PXD034105
     1. Label: PRIDE project
     2. Name: Characterising early phosphorylation events upon HER2 protein overexpression