PXD034100-1

PXD034100 is an original dataset announced via ProteomeXchange.

Title	CIP2A-TOPBP1 Interaction in Human Mitotic Cells
Description	We investigate the implications of DNA double strand breaks in the human genome, regarding the progression and treatment of various cancer types. During mitosis, the canonical DNA repair mechanisms are suspended while broken chromosomes get temporarily stabilized through so-called adapter proteins. We already knew that TOPBP1 is involved in that process and recently, we identified another Protein which recruits TOPBP1 to sites of damage. After mapping the interaction site on TOPBP1 we attempted to pull down CIP2A using a fragment of the binding domain, followed by chromatography-tandem-mass spectrometry analysis, the results of which we share in this upload.
HostingRepository	PRIDE
AnnounceDate	2022-07-19
AnnouncementXML	Submission_2022-07-19_01:52:37.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alain Jeanrenaud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 03:17:05	ID requested
⏵ 1	2022-07-19 01:52:37	announced
2	2023-11-14 08:56:59	announced	2023-11-14: Updated project metadata.

10.1038/S41467-022-31865-5;

submitter keyword: UZH, USZ, FGCZ, CIP2A, TOPBP1, Human, Cancer, Mitosis, 293T, Pull-Down, LC-MS/MS, nanoAcquity, UPLC, Q-Exactive

Manuel Guido Stucki
contact affiliation	University of Zürich / University Hospital Zürich, Switzerland, Clinic of Gynecology
contact email	manuel.stucki@uzh.ch
lab head
Alain Jeanrenaud
contact affiliation	University of Zürich
contact email	alain.jeanrenaud@usz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034100

PRIDE project URI

• PRIDE
  1. PXD034100
     1. Label: PRIDE project
     2. Name: CIP2A-TOPBP1 Interaction in Human Mitotic Cells