PXD034098-1

PXD034098 is an original dataset announced via ProteomeXchange.

Title	Cyclin A and Cks1 promote kinase consensus switching to non-proline directed CDK1 phosphorylation
Description	Ordered protein phosphorylation by CDKs is a key mechanism for regulating the cell cycle. How temporal order is enforced in mammalian cells remains unclear. Using a fixed cell kinase assay and phosphoproteomics, we studied how CDK1 activity and non-catalytic CDK1 subunits contribute to the choice of substrate and phosphorylation site. Increases in CDK1 activity alters substrate choice, with intermediate and low sensitivity CDK1 substrates enriched in DNA replication and mitotic functions, respectively. This activity dependence was shared between Cyclin A- and Cyclin B-CDK1. Cks1 has a proteome-wide role as an enhancer of multisite CDK1 phosphorylation. Contrary to the model of CDK1 as an exclusively proline-directed kinase, we show that Cyclin A and Cks1 promote non-proline directed phosphorylation, preferably on sites with a +3 lysine residue. Indeed, 70% of cell cycle regulated phosphorylations, where the kinase carrying out this modification has not been identified, are non-proline directed CDK1 sites.
HostingRepository	PRIDE
AnnounceDate	2023-02-17
AnnouncementXML	Submission_2023-02-17_05:39:42.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TonyLy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 03:10:31	ID requested
⏵ 1	2023-02-17 05:39:43	announced

Dataset with its publication pending

submitter keyword: cell cycle, cdk1, kinase, phosphoproteomics

TonyLy
contact affiliation	Centre for Gene Regulation and Expression University of Dundee
contact email	tly@dundee.ac.uk
lab head
TonyLy
contact affiliation	University of Dundee
contact email	tly@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD034098

PRIDE project URI

• PRIDE
  1. PXD034098
     1. Label: PRIDE project
     2. Name:   Cyclin A and Cks1 promote kinase consensus switching to non-proline directed CDK1 phosphorylation