PXD034087 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays |
Description | In this study, we developed a model system for the assessment of small molecule-protein interactions using intact cells treated with a commercially available highly specific mitogen-activated protein kinase (MEK) 1/2 inhibitor to prepare a set of unfractionated test samples labeled with tandem mass tags. Our objective was to improve qualitative and quantitative aspects of MS-TSA as well as its efficiency and accuracy. We evaluated individually and for the first time in combination ΦSDM, FAIMS, and SIILCC as an improved MS-based acquisition approach for thermal stability assays (iMAATSA). PSM-level filtering was preliminarily investigated as an approach to reduce melting curve variation and improve the accuracy of Tm measurements. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:46:14.370.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Clifford Phaneuf |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-25 02:39:35 | ID requested | |
1 | 2023-05-10 10:39:23 | announced | |
⏵ 2 | 2023-11-14 08:46:23 | announced | 2023-11-14: Updated project metadata. |
Publication List
Phaneuf CG, Aizikov K, Grinfeld D, Kreutzmann A, Mourad D, Lange O, Dai D, Zhang B, Belenky A, Makarov AA, Ivanov AR, Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays. Commun Chem, 6(1):64(2023) [pubmed] |
Keyword List
submitter keyword: CETSA, mass spectrometry, TPP, target identification, thermal stability assays, drug-protein interactions |
Contact List
Prof. Alexander Ivanov |
contact affiliation | Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States |
contact email | a.ivanov@northeastern.edu |
lab head | |
Clifford Phaneuf |
contact affiliation | sanofi |
contact email | clifford.phaneuf@sanofi.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034087
- Label: PRIDE project
- Name: Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays