PXD034079-1

PXD034079 is an original dataset announced via ProteomeXchange.

Title	Role of the DDI2 protease in activation of TCF11/NRF1 and cellular control
Description	DDI2 has been shown to cleave and activate the ER-bound transcription factor TCF11/NRF1 (NFE2L1), a master regulator of proteostasis maintenance in mammalian cells, to ensure expression of rescue factors including proteasome subunits upon proteotoxic stress. Recent studies have identified DDI1-like proteases as endoproteases that cleave polyubiquitinated substrates. The unusual nature of these aspartic proteases is further supported by the fact that they participate in DNA repair. Despite all these findings, nothing is known about the physiological role and functional complexity of DDI2 function in cells. We therefore generated a CRISR/Cas9 knock-out of DDI2 in the human endothelial cell line Ea.Hy926 and compared the total proteome of the parental cell line with that of the DDI2 knock-out line by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:45:51.677.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Venz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 02:19:56	ID requested
⏵ 1	2026-08-23 16:45:52	announced

Nedomova M, Haberecht-M, ü, ller S, M, ö, ller S, Venz S, Prochazkova M, Prochazka J, Sedlak F, Chawengsaksophak K, Hammer E, Kasparek P, Adamek M, Sedlacek R, Konvalinka J, Kr, ü, ger E, Grantz Saskova K, DDI2 protease controls embryonic development and inflammation via TCF11/NRF1. iScience, 27(10):110893(2024) [pubmed]

10.1016/j.isci.2024.110893;

submitter keyword: LC-MS/MS, EA.hy926 cells, DDI2

Simone Venz
contact affiliation	University Medicine Greifswald, Institute of Medical Biochemistry and Molecular Biology
contact email	simone.venz@med.uni-greifswald.de
lab head
Simone Venz
contact affiliation	Institute of Medical Biochemistry and Molecular Biology - University Medicine Greifswald
contact email	simone.venz@med.uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034079
     1. Label: PRIDE project
     2. Name: Role of the DDI2 protease in activation of TCF11/NRF1 and cellular control