PXD034026-1

PXD034026 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A cell type-specific proteomics uncovers a guard cell-specific RAF15-SnRK2.6/OST1 kinase cascade
Description	Multicellular organisms such as plants contain different cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances understanding of organization and function at the organismal level. Here we used enzymatic isolation and flow cytometry-activated cell sorting (FACS) to enrich the guard cell and mesophyll cell protoplasts and acquire in-depth proteomics in these two major cell types in Arabidopsis leaves. We uncovered a guard cell-specific kinase cascade whereby RAF15 and OST1 mediate ABA-induced stomatal closure. This pipeline can be applied to various cell types in plant or non-plant systems to learn how cells function in highly organized multicellular organisms.
HostingRepository	jPOST
AnnounceDate	2023-05-23
AnnouncementXML	Submission_2023-05-22_08:00:04.436.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongliang Wang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxymethyl-L-cysteine; alpha-amino acetylated residue
Instrument	Orbitrap Fusion; instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-23 03:33:38	ID requested
⏵ 1	2023-05-22 08:00:04	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: proteomics, single-cell, guard cell, abscisic acid, phosphorylation

submitter keyword: proteomics, single-cell, guard cell, abscisic acid, phosphorylation

Contact List

Pengcheng Wang
lab head
Hongliang Wang
contact affiliation	Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001596/