PXD034014-1

PXD034014 is an original dataset announced via ProteomeXchange.

Title	The small RNA Teg41 is a pleiotropic regulator of virulence in Staphylococcus aureus
Description	Staphylococcus aureus is Gram-positive commensal bacteria that can also cause human disease ranging from mild, self-resolving skin infections to life-threatening conditions like endocarditis, osteomyelitis, and septicemia. Previously we demonstrated a role for the S. aureus sRNA, Teg41 in regulating production of the alpha Phenol Soluble Modulin toxins (αPSMs). In this study, we further characterize the regulatory role of Teg41. RNAseq analysis shows Teg41 influences the abundance of not just the αPSM transcript, but a variety of other transcripts as well compared to wild type S. aureus. Proteomic analysis confirms that eliminating Teg41 from the cell influences both the cytoplasmic and secreted protein profile of S. aureus. Finally, we observe that the Teg41Δ3′ strain is more severely attenuated in two murine infection models than a ∆αPSM strain, indicating that Teg41 regulation reaches beyond the αPSMs. Overall, we show that Teg41 is a unique pleiotropic sRNA in S. aureus that influences several key cellular processes.
HostingRepository	PRIDE
AnnounceDate	2022-10-03
AnnouncementXML	Submission_2022-10-03_06:13:44.121.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ronan Carroll
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-20 10:43:26	ID requested
⏵ 1	2022-10-03 06:13:44	announced

Dataset with its publication pending

submitter keyword: Staphylococcus aureus Teg41 PSM spa

Ronan Carroll
contact affiliation	Department of Biological Sciences, Ohio University
contact email	carrolr3@ohio.edu
lab head
Ronan Carroll
contact affiliation	Ohio University
contact email	carrolr3@ohio.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034014
     1. Label: PRIDE project
     2. Name: The small RNA Teg41 is a pleiotropic regulator of virulence in Staphylococcus aureus