PXD033976-1

PXD033976 is an original dataset announced via ProteomeXchange.

Title	In situ Analysis of Nanoparticle Soft Corona and Dynamic Evolution
Description	How soft corona i.e., the protein corona’s outer layer contributes to “biological” identity of nanomaterials (NMs) is largely unknown due to a longstanding challenge in capturing protein composition of the soft corona in situ. We herein develop an in situ “Fishing” method that can monitor the dynamic formation of protein corona on ultra-small chiral Cu2S nanoparticles (NPs) allowing us to directly separate and identify their protein composition. This method can detect spatiotemporal processes in the evolution of hard and soft coronas on chiral NPs, revealing subtle differences in their composition even within several minutes. This study highlights the importance of in situ and dynamic analysis of soft/hard corona composition, provides detailed insights into the roles of protein corona composition in mediating the biological responses of NPs, and offers a universal strategy to characterize the soft corona that will serve as a powerful tool for rational design of biomedical NMs.
HostingRepository	PRIDE
AnnounceDate	2022-10-13
AnnouncementXML	Submission_2022-10-13_03:35:31.768.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LimingWang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 07:32:33	ID requested
⏵ 1	2022-10-13 03:35:32	announced
2	2023-11-14 07:12:30	announced	2023-11-14: Updated project metadata.

Baimanov D, Wang J, Zhang J, Liu K, Cong Y, Shi X, Zhang X, Li Y, Li X, Qiao R, Zhao Y, Zhou Y, Wang L, Chen C, In situ analysis of nanoparticle soft corona and dynamic evolution. Nat Commun, 13(1):5389(2022) [pubmed]

submitter keyword: protein corona,mouse serum

ChunyingChen
contact affiliation	CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS-HKU Joint Laboratory of Metallomics on Health and Environment & CAS Center for Excellence in Nanoscience, Institute of High Energy Physics & National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, Beijing 100049, P. R. China
contact email	chenchy@nanoctr.cn
lab head
LimingWang
contact affiliation	Institute of High Energy Physics, Chinese Academy of Sciences
contact email	wangliming@ihep.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033976

PRIDE project URI

• PRIDE
  1. PXD033976
     1. Label: PRIDE project
     2. Name: In situ Analysis of Nanoparticle Soft Corona and Dynamic Evolution