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PXD033976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn situ Analysis of Nanoparticle Soft Corona and Dynamic Evolution
DescriptionHow soft corona i.e., the protein corona’s outer layer contributes to “biological” identity of nanomaterials (NMs) is largely unknown due to a longstanding challenge in capturing protein composition of the soft corona in situ. We herein develop an in situ “Fishing” method that can monitor the dynamic formation of protein corona on ultra-small chiral Cu2S nanoparticles (NPs) allowing us to directly separate and identify their protein composition. This method can detect spatiotemporal processes in the evolution of hard and soft coronas on chiral NPs, revealing subtle differences in their composition even within several minutes. This study highlights the importance of in situ and dynamic analysis of soft/hard corona composition, provides detailed insights into the roles of protein corona composition in mediating the biological responses of NPs, and offers a universal strategy to characterize the soft corona that will serve as a powerful tool for rational design of biomedical NMs.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiming Wang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-18 07:32:33ID requested
12022-10-13 03:35:32announced
22023-11-14 07:12:30announced2023-11-14: Updated project metadata.
Publication List
Baimanov D, Wang J, Zhang J, Liu K, Cong Y, Shi X, Zhang X, Li Y, Li X, Qiao R, Zhao Y, Zhou Y, Wang L, Chen C, In situ analysis of nanoparticle soft corona and dynamic evolution. Nat Commun, 13(1):5389(2022) [pubmed]
Keyword List
submitter keyword: protein corona,mouse serum
Contact List
Chunying Chen
contact affiliationCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS-HKU Joint Laboratory of Metallomics on Health and Environment & CAS Center for Excellence in Nanoscience, Institute of High Energy Physics & National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, Beijing 100049, P. R. China
contact emailchenchy@nanoctr.cn
lab head
Liming Wang
contact affiliationInstitute of High Energy Physics, Chinese Academy of Sciences
contact emailwangliming@ihep.ac.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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