PXD033951-3

PXD033951 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the human hippocampus identifies neuronal pentraxin 1 (NPTX1) as synapto-axonal target in late-stage Parkinson's disease
Description	Parkinson's disease (PD) affects a significant proportion of the population over the age of 60 years, and its prevalence is increasing. While symptomatic treatment is available for motor symptoms of PD, non-motor complications such as dementia result in diminished life quality for patients and are far more difficult to treat. In this study, we analyzed PD-associated alterations in the hippocampus of PD patients, since this brain region is strongly affected by PD dementia. We focused on synapses, analyzing the proteome of post-mortal hippocampal tissue from 16 PD cases and 14 control subjects by mass spectrometry. Whole tissue lysates and synaptosomal fractions were analyzed in parallel. Differential analysis combined with bioinformatic network analyses identified neuronal pentraxin 1 (NPTX1) to be significantly dysregulated in PD and interacting with proteins of the synaptic compartment. Modulation of NPTX1 protein levels in primary hippocampal neuron cultures validated its role in synapse morphology. Our analysis suggests that NPTX1 contributes to synaptic pathology in late-stage PD and represents a putative target for novel therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:54.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henning Urlaub
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 04:01:11	ID requested
1	2023-11-12 15:36:57	announced
2	2023-11-14 07:47:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:54	announced	2024-10-22: Updated project metadata.

10.1111/JNC.15924;

submitter keyword: Parkinson’s Disease

neurodegeneration

proteomics

synaptic dysfunction

NPTX1

TMT

Prof. Dr.
contact affiliation	Bioanalytical Mass Spectrometry Group, Max-Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Goettingen; Institute for Clinical Chemistry, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen
contact email	henning.urlaub@mpinat.mpg.de
lab head
Henning Urlaub
contact affiliation	Max-Plank Institute for Biophysical Chemistry
contact email	msbio.goettingen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD033951

PRIDE project URI

• PRIDE
  1. PXD033951
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the human hippocampus identifies neuronal pentraxin 1 (NPTX1) as synapto-axonal target in late-stage Parkinson's disease