PXD033940-1

PXD033940 is an original dataset announced via ProteomeXchange.

Title	An optimized proteomics approach reveals novel alternative proteins in mouse liver development
Description	Alternative open reading frames (AltORFs) are unannotated sequences in genome that encode novel peptides or proteins named alternative proteins (AltProts). Although ribosome profiling and bioinformatics predict a large number of AltProts, mass spectrometry (MS) as the only direct way of identification, is hampered by the short lengths and relative low abundance of AltProts. Here we report an approach based on size-exclusion chromatography (SEC) for simultaneous enrichment and fractionation of AltProts from mouse livers. With this strategy we compared and discovered 89 novel AltProts between embryonic and adult mouse livers and will extend our optimized workflow in various disease models in future study.
HostingRepository	PRIDE
AnnounceDate	2022-12-23
AnnouncementXML	Submission_2022-12-23_04:37:46.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YingYANG
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-17 08:28:28	ID requested
⏵ 1	2022-12-23 04:37:47	announced
2	2023-11-14 08:56:08	announced	2023-11-14: Updated project metadata.

submitter keyword: liver development., mass spectrometry, fractionation,Alternative open reading frames (AltORFs), alternative proteins (AltProts), enrichment

QianZhao
contact affiliation	State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, SAR, China
contact email	q.zhao@polyu.edu.hk
lab head
YingYANG
contact affiliation	the Hong Kong Polytechnic University
contact email	20033267r@connect.polyu.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033940
     1. Label: PRIDE project
     2. Name: An optimized proteomics approach reveals novel alternative proteins in mouse liver development