PXD033930-2

PXD033930 is an original dataset announced via ProteomeXchange.

Title	KLF16 enhances stress tolerance of colorectal carcinomas by modulating nucleolar homeostasis and translational reprogramming
Description	Translational reprogramming is part of the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress, which acts to the advantage of cancer growth and development in different stress conditions. But the mechanism of ER stress-related translational reprogramming in colorectal carcinoma (CRC) progression remains unclear. Here, we identified that Krüppel-Like Factor 16 (KLF16) can promote CRC progression and stress tolerance through translational reprogramming. The expression of KLF16 was upregulated in CRC tissues and associated with poor prognosis for CRC patients. We found that ER stress inducers can recruit KLF16 to the nucleolus and increase its interaction with two essential proteins for nucleolar homeostasis, nucleophosmin1 (NPM1) and fibrillarin (FBL). Moreover, knockdown of KLF16 can dysregulate nucleolar homeostasis in CRC cells. Translation-reporter system and polysome profiling assays further showed that KLF16 can effectively promote cap-independent translation of ATF4, which can enhance ER-phagy and proliferation of CRC cells. Overall, our study unveils a previously unrecognized role for KLF16 as an ER stress regulator through mediating translational reprogramming to enhance stress tolerance of CRC cells and provides a potential therapeutic vulnerability.
HostingRepository	iProX
AnnounceDate	2022-05-16
AnnouncementXML	Submission_2023-08-14_00:33:19.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaodan Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 20:00:09	ID requested
1	2022-05-16 20:00:16	announced
⏵ 2	2023-08-14 00:33:20	announced	2023-08-14: Update publication information.

Ma XD, Xu SD, Hao SH, Han K, Chen JW, Ling H, Chen RX, Jin XH, Cao JH, Lin JL, Ou QJ, Fang YJ, Pan ZZ, Xie D, Wang FW, KLF16 enhances stress tolerance of colorectal carcinomas by modulating nucleolar homeostasis and translational reprogramming. Mol Ther, 30(8):2828-2843(2022) [pubmed]

submitter keyword: carcinogenesis, ER stress, stress tolerance, nucleolar homeostasis, translational reprogramming

Fengwei Wang
contact affiliation	Sun Yat-sen University
contact email	wangfengw@sysucc.org.cn
lab head
Xiaodan Ma
contact affiliation	Sun Yat-sen University
contact email	maxd@sysucc.org.cn
dataset submitter

iProX dataset URI