PXD033923-1

PXD033923 is an original dataset announced via ProteomeXchange.

Title	The Human Nse5 Orthologue SIMC1 Localizes SMC5/6 to Polyomavirus Replication Centers
Description	The human SMC5-SMC6 complex (SMC5/6) is a conserved guardian of genome stability and an emerging component of the antiviral response in animals and plants. In yeast, Smc5/6 is recruited to and loaded on chromatin by the poorly conserved Nse5-Nse6 heterodimer (Nse5/6), which remains largely undefined in humans. Here we report that SIMC1 is an Nse5 orthologue and novel subunit of the human SMC5/6 complex. SIMC1 binds to SLF2, the proposed Nse6 orthologue, forming a stable complex structurally similar to yeast Nse5/6. SIMC1 localizes SMC5/6 to polyomavirus replication centers in a SUMO interaction-dependent manner. Further structure prediction analyses indicate that SLF1, a putative Nse5 counterpart that recruits SMC5/6 to DNA lesions, interacts with SLF2 in a very similar manner as SIMC1. Consistently, SIMC1 and SLF1 form mutually exclusive complexes with SLF2. Thus, both SIMC1 and SLF1 act as orthologues of yeast Nse5 to independently direct the functions of SMC5/6.
HostingRepository	PRIDE
AnnounceDate	2022-11-29
AnnouncementXML	Submission_2022-11-29_07:19:00.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YasamanJami-Alahmadi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 08:25:36	ID requested
⏵ 1	2022-11-29 07:19:00	announced

Dataset with its publication pending

submitter keyword: Nse5, LCMS, SIMC1 Localizes SMC5/6

Michael NicholasBoddy
contact affiliation	Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
contact email	nboddy@scripps.edu
lab head
YasamanJami-Alahmadi
contact affiliation	UCLA
contact email	yjami@ucla.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD033923

PRIDE project URI

• PRIDE
  1. PXD033923
     1. Label: PRIDE project
     2. Name: The Human Nse5 Orthologue SIMC1 Localizes SMC5/6 to Polyomavirus Replication Centers