PXD033910-1

PXD033910 is an original dataset announced via ProteomeXchange.

Title	Elucidation of the role of murine lncRNA LL35 in hepatocytes by LC-MSMS
Description	Long non-coding RNAs (lncRNAs) play important roles in the regulation of many biological processes in the cell like transcription, translation, splicing, transport, etc. More than 10K lncRNAs are predicted in human, but functions for the majority remain unknown. LncRNAs may contribute to the development of multiple diseases that makes them perspective diagnostic and prognostic markers as well as drug targets [1]. Murine lncRNA Falcor/LL35 is a proposed functional analog of human lncRNA DEANR1, which is predominantly expressed in murine lungs and liver, intestine and pancreas [2, 3]. While the participation of LL35 in LL35–Foxa2 (transcription factor) regulatory loop during regeneration after lung injury was previously described by Swarr et al. [2], its functional role in murine liver remains unknown. In our work we focused on revealing the role of murine lncRNA LL35 in hepatocytes and in mouse liver. We analyzed changes in proteome of AML12 (normal hepatocytes) cell line on the 2nd day after LL35 depletion. [1] Morlando, M., Ballarino, M., & Fatica, A. (2015). Long non-coding RNAs: New players in hematopoiesis and leukemia. Frontiers in Medicine, Vol. 2. [2] Swarr, D. T., Herriges, M., Li, S., Morley, M., Fernandes, S., Sridharan, A., … Morrisey, E. E. (2019). The long noncoding RNA Falcor regulates Foxa2 expression to maintain lung epithelial homeostasis and promote regeneration. Genes & Development, 33(11-12), 656–668. [3] Sergeeva, O. V, Korinfskaya, S. A., Kurochkin, I. I., & Zatsepin, T. S. (2019). Long Noncoding RNA LL35 / Falcor Regulates Expression of Transcription Factor Foxa2 in Hepatocytes in Normal and Fibrotic Mouse Liver. Acta Naturae, 11(42), 66–74.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_04:12:39.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rustam Ziganshin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 06:57:42	ID requested
⏵ 1	2022-08-11 04:12:40	announced

Shcherbinina E, Abakumova T, Bobrovskiy D, Kurochkin I, Deinichenko K, Stekolshchikova E, Anikanov N, Ziganshin R, Melnikov P, Khrameeva E, Logacheva M, Zatsepin T, Sergeeva O, Murine Falcor/LL35 lncRNA Contributes to Glucose and Lipid Metabolism In Vitro and In Vivo. Biomedicines, 10(6):(2022) [pubmed]

submitter keyword: AML12 cells, murine lncRNA LL35 , LC-MSMS

Timofei S. Zatsepin
contact affiliation	Department of Chemistry, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia
contact email	t.zatsepin@skoltech.ru
lab head
Rustam Ziganshin
contact affiliation	Shemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences
contact email	rustam.ziganshin@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033910
     1. Label: PRIDE project
     2. Name: Elucidation of the role of murine lncRNA LL35 in hepatocytes by LC-MSMS