PXD033906-1

PXD033906 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of extracellular vesicles derived from wildtype or mutant EMCV-infected cells
Description	Non-enveloped viruses, such as picornaviruses, can escape infected host cells via release in extracellular vesicles (EVs) prior to the induction of lysis. These EVs cloak the secreted virus particles in a host-derived membrane, which alters virus-host interactions that affect infection efficiency and antiviral immunity. Currently, little is known about the viral and host factors regulating the formation and release of EV-enclosed viruses. The encephalomyocarditis virus (EMCV), belonging to the family of picornaviruses, expresses a Leader protein that targets various host cell processes. In this study, LC-MS/MS proteomic analysis was performed on EVs isolated via density gradient-based separation from HeLa cells infected with wildtype or Leader-deficient EMCV. Proteomes of these EVs were compared and proteins incorporated in EVs in a Leader-dependent manner were subjected to functional enrichment analysis and protein-interaction analysis.
HostingRepository	PRIDE
AnnounceDate	2022-06-02
AnnouncementXML	Submission_2022-06-02_05:56:04.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maarten Altelaar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 06:50:16	ID requested
⏵ 1	2022-06-02 05:56:05	announced

Dataset with its publication pending

submitter keyword: extracellular vesicles (EVs) , picornaviruses, LC-MS

Maarten Altelaar
contact affiliation	Utrecht University
contact email	m.altelaar@uu.nl
lab head
Maarten Altelaar
contact affiliation	Utrecht University
contact email	m.altelaar@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD033906

PRIDE project URI

• PRIDE
  1. PXD033906
     1. Label: PRIDE project
     2. Name: Comparative proteomics of extracellular vesicles derived from wildtype or mutant EMCV-infected cells