PXD033843-1

PXD033843 is an original dataset announced via ProteomeXchange.

Title	Duplicated ribosomal protein paralogs promote alternative translation and drug resistance
Description	Ribosomes are often seen as monolithic machines produced from uniformly regulated genes. However, in yeast most ribosomal proteins come from duplicated genes. Here, we demonstrate that gene duplication may serve as a stress-adaptation mechanism modulating the global proteome through the differential expression of ribosomal protein paralogs. Our data indicate that the yeast paralog pair of the ribosomal protein L7/uL30 produces two differentially acetylated proteins. Under normal conditions most ribosomes incorporate the hypo-acetylated major form favoring the translation of genes with short open reading frames. Exposure to drugs, on the other hand, increases the production of ribosomes carrying the hyper-acetylated minor paralog that increases translation of long open reading frames. Many of these paralogs dependent genes encode cell wall proteins that could promote tolerance to drugs as their translation increases after exposure to drugs. Together the data reveal a mechanism of translation control through the differential use of near-identical ribosomal protein isoforms.
HostingRepository	PRIDE
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2022-08-09_11:50:42.301.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathieu Catala
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 04:29:44	ID requested
⏵ 1	2022-08-09 11:50:42	announced

Dataset with its publication pending

submitter keyword: Saccharomyces cerevisiae, dRPG, paralogs

Sherif Abou Elela
contact affiliation	Universite de Sherbrooke
contact email	sherif.abou.elela@usherbrooke.ca
lab head
Mathieu Catala
contact affiliation	Universite de Sherbrooke
contact email	mathieu.catala@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033843
     1. Label: PRIDE project
     2. Name: Duplicated ribosomal protein paralogs promote alternative translation and drug resistance